MaxTwo 高通量高分辨率細(xì)胞電刺激培養(yǎng)成像儀

MaxTwo高通量高分辨率細(xì)胞電刺激培養(yǎng)成像儀

MaxTwo, a high-throughput, high-resolution electrophysiology platform for recording and stimulating electrogenic cells in vitro. MaxTwo combines 6 or 24 MaxOne high-density microelectrode arrays in a multiwell format.

Product Overview

MaxWell Biosystems’ MaxTwo is a CMOS-based multiwell HD-MEA platform. It has all the capabilities of MaxOne–it captures high-quality signals at sub cellular resolution from long-term culture experiments. Electrical stimulation protocols can also be performed.

MaxTwo multiwell HD-MEA system allows for easy and vibration-free insertion of the MEA device. It can operate inside cell-culture incubators and is designed for integration with automation systems. Microscopy of samples in MaxTwo wells can be performed using upright objectives. Overall, MaxTwo is a powerful electrical imaging system for drug discovery, safety pharmacology, and iPSC phenotyping applications.

Key Features per Well

• 26,400 electrodes (9.3×5.45 sq-μm, 17.5 μm pitch)
• 1024 low-noise readout channels
• 32 stimulation channels
• Large sensor area (3.85×2.10 sq-mm)
• Switch-matrix technology for flexible array reconfiguration
• Non-invasive, label-free
• High-resolution activity map
• Axonal action potential propagation tracking
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MaxOne是記錄和刺激體外細(xì)胞的zui強(qiáng)大的電生理平臺(tái)。 MaxOne是單孔格式的高密度微電極陣列（HD-MEA）系統(tǒng)。
MaxOne是一款基于CMOS的高密度微電極陣列（HD-MEA）系統(tǒng)。
這使得緊湊而又功能強(qiáng)大的放大器，濾波器和數(shù)字化儀能夠集成到MEA中，并且與待研究的細(xì)胞緊密相連。

?高分辨率，無(wú)標(biāo)記細(xì)胞活動(dòng)成像
?亞細(xì)胞和網(wǎng)絡(luò)活動(dòng)的無(wú)標(biāo)記檢測(cè)
?多種選擇性電刺激
?全面的數(shù)據(jù)分析工具

從原發(fā)組織中收集細(xì)胞，如大腦，視網(wǎng)膜或心臟。

準(zhǔn)備實(shí)驗(yàn)時(shí)，開(kāi)始在MaxWell Biosystems的MEA芯片上培養(yǎng)細(xì)胞。

開(kāi)始您的實(shí)驗(yàn)：在MaxLab Live軟件的幫助下，跟蹤結(jié)果并在計(jì)算機(jī)上分析數(shù)據(jù)。

使用結(jié)果來(lái)指導(dǎo)進(jìn)一步的實(shí)驗(yàn)，獲得科學(xué)見(jiàn)解并發(fā)布數(shù)據(jù)。

Multiscale recording and analysis —

1. High-resolution, label-free cell activity imaging

Analyze subcellular features, such as full axonal arbors in single neurons.

Electrical signals tracked along neurites enable to investigate novel parameters. High-resolution tracking of axonal action potential propagation allows for investigating changes in axonal conduction velocity.

2. Whole sample activity map

Localize active cells on the MEA during experiments.

The morphological structure of a primary cortical cell culture was imaged using an upright microscope with 10x objective. MAP2 staining was used to visualize the neurons. The optical image closely matches the electrical image obtained using MaxOne. The electrical image provides the location of the cells, as well as information on the activity of the cells, such as spike rate and amplitude.

3. Smart population recording

Identify defined cells for long term recording and further analyses.

Different combinations of parameters can be used to automatically select a defined set of cells to be recorded, such as spike rate, amplitude, etc. The raster plot shows the dynamics of the network activity using 1,024 active electrode sites. Burst features can be investigated in detail (one burst zoomed in).