HEXAGON-IMAGING-PAM 蜂巢矩陣葉綠素?zé)晒獬上裣到y(tǒng)

蜂巢矩陣葉綠素?zé)晒獬上裣到y(tǒng)

HEXAGON-IMAGING-PAM

葉綠素?zé)晒獬上裣到y(tǒng)的“六邊形戰(zhàn)士”

精度高，面積大，功能全，應(yīng)用廣，文獻(xiàn)多，數(shù)據(jù)可視化！

HEXAGON-IMAGING-PAM是德國(guó)WALZ公司最新推出的大型蜂巢矩陣葉綠素?zé)晒獬上裣到y(tǒng)。它憑借高精度的脈沖振幅調(diào)制（PAM）技術(shù)，可以對(duì)20×24cm的區(qū)域進(jìn)行成像。分辨率高達(dá)1.2 MP（1000 x 1200 px, 2x2 binning技術(shù)，實(shí)際是2000×2400），像素尺寸3.45 x 3.45 µm。

超高分辨率的基礎(chǔ)是成像區(qū)域光場(chǎng)的均勻性，在設(shè)計(jì)過(guò)程中，光源陣列中LED的位置是經(jīng)過(guò)精心布局的，以保證測(cè)量區(qū)域內(nèi)無(wú)陰影，所有成像區(qū)域內(nèi)的樣品均勻照光，樣品間的差異可以盡收眼底。大功率LED面板的冷卻效果非常好，可以最大限度的延長(zhǎng)LED的使用壽命。

增加遠(yuǎn)紅光（FR）LED 面板，可用于測(cè)量所研究樣品的Fo'值。

HEXAGON-IMAGING-PAM采用蜂巢矩陣式LED面板拼接技術(shù)，單個(gè)六邊形蜂巢矩陣單元之間LED的不平橫可以獨(dú)立補(bǔ)償，初衷是為實(shí)現(xiàn)樣品區(qū)域的理想照明提供更優(yōu)選擇。

盡管成像區(qū)域很大，但是它依然足夠靈活，可以測(cè)量各種類型的樣品，如盆栽植物，穴盤中培養(yǎng)的植物，培養(yǎng)皿上的植物或多孔板中的藻類懸浮液。

滑動(dòng)門設(shè)計(jì)，集成安全關(guān)閉功能，開門狀態(tài)下，飽和脈沖的強(qiáng)度會(huì)被抑制以保護(hù)操作人員的眼睛。

主要功能

l 原位測(cè)量：活體植物葉綠素?zé)晒獬上?，直觀顯示樣品光合作用光能利用差異，可導(dǎo)出彩色圖像。

l 成像功能：對(duì)Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、PS/50=ETR、Inh等參數(shù)進(jìn)行成像分析。測(cè)定調(diào)節(jié)性能量耗散Y(NPQ)，反映植物光保護(hù)能力，測(cè)定非調(diào)節(jié)性能量耗散Y(NO)，反映植物光損傷程度。

l 程序測(cè)量功能：可自動(dòng)程序測(cè)量熒光誘導(dǎo)曲線、快速光曲線和暗弛豫，也可手動(dòng)測(cè)量；在測(cè)量過(guò)程中能自動(dòng)分析所有熒光參數(shù)的變化趨勢(shì)；可以預(yù)編程進(jìn)行自定義實(shí)驗(yàn)流程，如模擬波動(dòng)光。

l AOI功能：可在測(cè)量前或測(cè)量后任意選擇感興趣的區(qū)域（AOI），程序?qū)⒆詣?dòng)對(duì)選擇的AOI的數(shù)據(jù)進(jìn)行變化趨勢(shì)分析，并在報(bào)告文件中顯示相關(guān)AOI的數(shù)據(jù)。所有報(bào)告文件中顯示的數(shù)據(jù)都可導(dǎo)出到EXCEL文件中。

l 成像異質(zhì)性分析功能：對(duì)任意參數(shù)任意時(shí)間的成像，可在圖像上任意選取兩點(diǎn)，軟件自動(dòng)對(duì)兩點(diǎn)間的數(shù)據(jù)進(jìn)行橫向異質(zhì)性分析，并可導(dǎo)出到EXCEL文件中。

l 成像數(shù)據(jù)范圍分析功能：對(duì)任意參數(shù)任意時(shí)間的成像，可分析任意兩個(gè)熒光數(shù)值之間有多少個(gè)像素點(diǎn)，多少面積（cm2）。

l 突變株篩選功能：可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。

l 微藻毒理研究功能：可同時(shí)測(cè)量4塊96孔板，即384個(gè)微藻樣品（對(duì)照和處理組）的光合活性，軟件自動(dòng)給出處理組樣品相對(duì)于對(duì)照組的光合抑制百分比。

應(yīng)用領(lǐng)域

l 光合作用研究：可以在*相同的條件下同時(shí)對(duì)大量樣品進(jìn)行成像

l 植物病理學(xué)：病斑部位（包括肉眼不可見(jiàn)時(shí)）成像以及病斑擴(kuò)散的時(shí)空動(dòng)力學(xué)

l 植物脅迫生理學(xué)：肉眼不可見(jiàn)生物/非生物脅迫損傷的早期檢測(cè)

l 遺傳育種：出苗后大規(guī)模快速篩選高光合/抗旱/抗熱/抗凍/抗病等植株

l 突變株篩選：快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等

l 微藻毒理學(xué)：不同毒物濃度多個(gè)重復(fù)的樣品一次測(cè)完，軟件自動(dòng)計(jì)算抑制比率

l 其它多種擴(kuò)展研究

成像參數(shù)

Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), PS/50=ETR,Inh.等

產(chǎn)地：德國(guó)WALZ

參考文獻(xiàn)

數(shù)據(jù)來(lái)源：光合作用文獻(xiàn)Endnote數(shù)據(jù)庫(kù)，原始數(shù)據(jù)來(lái)源：Google Scholar。

注：HEXAGON-IMAGING-PAM為最新產(chǎn)品，暫無(wú)文獻(xiàn)發(fā)表，最新研究成果可參考M-IMAGING-PAM發(fā)表文章。

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014年2月，上海澤泉科技股份有限公司在上海浦東孫橋現(xiàn)代農(nóng)業(yè)園區(qū)投資成立了上海乾菲諾農(nóng)業(yè)科技有限公司，建設(shè)了AgriPhenoTM “高通量植物基因型-表型-育種服務(wù)平臺(tái)”，為植物科研和育種單位提供全面的樣品收集和栽培，實(shí)驗(yàn)設(shè)計(jì)和項(xiàng)目合作，以及表型數(shù)據(jù)與生物信息學(xué)分析綜合服務(wù)。平臺(tái)成功主持了上海張江國(guó)家自主創(chuàng)新示范區(qū)專項(xiàng)發(fā)展資金重點(diǎn)項(xiàng)目“澤泉科技高通量植物基因型-表型-育種服務(wù)平臺(tái)”。作為主持單位或合作單位參與了上海市農(nóng)委和科委的30多項(xiàng)政府科研服務(wù)項(xiàng)目以及商業(yè)服務(wù)項(xiàng)目，如科技興農(nóng)種業(yè)發(fā)展項(xiàng)目“農(nóng)作物分子育種的技術(shù)創(chuàng)新研究”和“青菜高通量表型圖譜標(biāo)準(zhǔn)的建立及主要性狀分析”、科技興農(nóng)重點(diǎn)攻關(guān)項(xiàng)目“基于圖像分析及三維建模技術(shù)的黃瓜長(zhǎng)勢(shì)快速評(píng)價(jià)方法研究”、 “蘭科觀賞花卉分子育種技術(shù)研究與產(chǎn)業(yè)化應(yīng)用”等。為了緊追世界科技發(fā)展水平，開啟院企合作建立研究型平臺(tái)的創(chuàng)新嘗試，上海澤泉科技股份有限公司與上海市農(nóng)業(yè)科學(xué)院，結(jié)合雙方各自的優(yōu)勢(shì)，于2021年5月在上海農(nóng)業(yè)科學(xué)院莊行試驗(yàn)站聯(lián)合成立“上海市農(nóng)業(yè)科學(xué)院莊行綜合試驗(yàn)站澤泉科技植物表型技術(shù)研究平臺(tái)”，AgriPhenoTM平臺(tái)從上海浦東孫橋現(xiàn)代農(nóng)業(yè)園區(qū)整體遷出，并入新建的植物表型技術(shù)研究平臺(tái)。目前平臺(tái)除擁有無(wú)人機(jī)表型平臺(tái)、溫室型和實(shí)驗(yàn)室型高通量表型分析系統(tǒng)外，還擁有現(xiàn)代化溫室、生物學(xué)實(shí)驗(yàn)室、植物生理生態(tài)測(cè)量設(shè)備、農(nóng)業(yè)氣象測(cè)量系統(tǒng)和專業(yè)的數(shù)據(jù)庫(kù)平臺(tái)，已經(jīng)具備了對(duì)植物、動(dòng)物基因測(cè)序與植物表型研究的各類條件?？梢猿袚?dān)高通量DNA提取、基因測(cè)序服務(wù)、分子輔助育種、植物生理生態(tài)研究等科研實(shí)驗(yàn)任務(wù)。同時(shí)可以為植物功能基因組、農(nóng)業(yè)育種家提供高通量植物基因型測(cè)試、高通量植物表型測(cè)試和植物基因型-表型生物信息學(xué)數(shù)據(jù)分析等開放式服務(wù)。