Dxy GeneArt™ Gibson Assembly HiFi 預混液
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- 公司名稱 賽默飛世爾科技（中國）有限公司
- 品牌 Invitrogen
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- 廠商性質 生產(chǎn)廠家
- 更新時間 2024/12/18 11:27:12
- 訪問次數(shù) 53
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賽默飛世爾科技賽默飛世爾科技（紐約證交所代碼：TMO）是科學服務領域的世界領導者。公司年銷售額超過250億美元。在全球擁有約70,000名員工。我們的使命是攜手客戶，讓世界更健康、更清潔、更安全。我們幫助客戶加速生命科學領域的研究、解決在分析領域所遇到的復雜問題與挑戰(zhàn)，促進醫(yī)療診斷發(fā)展、加速藥物上市進程、提高實驗室生產(chǎn)力。我們?nèi)虺^75,000名賽默飛員工將借助于一系列行業(yè)領先的品牌Thermo Scientific、Applied Biosystems、Invitrogen、Fisher Scientific、Unity Lab Services和Patheon，為客戶提供另想的創(chuàng)新技術、便捷采購方案和全方位服務。
欲了解更多信息，請瀏覽公司網(wǎng)站：www.thermofisher.com 賽默飛世爾科技中國簡介賽默飛世爾科技進入中國發(fā)展已超過35年，在中國的總部設于上海，并在北京、廣州、香港、成都、沈陽、西安、南京、武漢、昆明等地設立了分公司，員工人數(shù)約為5000名。我們的產(chǎn)品主要包括分析儀器、實驗室設備、試劑、耗材和軟件等，提供實驗室綜合解決方案，為各行各業(yè)的客戶服務。為了滿足中國市場的需求，現(xiàn)有7家工廠分別在上海、北京、蘇州和廣州等地運營。我們在全國還設立了8個應用開發(fā)中心以及示范實驗室，將世界級的前沿技術和產(chǎn)品帶給中國客戶，并提供應用開發(fā)與培訓等多項服務；擁位于上海的中國創(chuàng)新中心，擁有有100多位專業(yè)研究人員和工程師及70多項專利。創(chuàng)新中心專注于針對垂直市場的產(chǎn)品研究和開發(fā)，結合中國市場的需求和國外先進技術，研發(fā)適合中國的技術和產(chǎn)品；我們擁有遍布全國的維修服務網(wǎng)點和特別成立的中國技術培訓團隊，在全國有超過2600名專業(yè)人員直接為客戶提供服務。我們致力于幫助客戶使世界更健康、更清潔、更安全。
欲了解更多信息，請登錄網(wǎng)站：www.thermofisher.com 全國服務熱線：021-61621892公司電子郵箱：sales.china@thermofisher.com
欲了解更多信息，請瀏覽公司網(wǎng)站：www.thermofisher.com 賽默飛世爾科技中國簡介賽默飛世爾科技進入中國發(fā)展已超過35年，在中國的總部設于上海，并在北京、廣州、香港、成都、沈陽、西安、南京、武漢、昆明等地設立了分公司，員工人數(shù)約為5000名。我們的產(chǎn)品主要包括分析儀器、實驗室設備、試劑、耗材和軟件等，提供實驗室綜合解決方案，為各行各業(yè)的客戶服務。為了滿足中國市場的需求，現(xiàn)有7家工廠分別在上海、北京、蘇州和廣州等地運營。我們在全國還設立了8個應用開發(fā)中心以及示范實驗室，將世界級的前沿技術和產(chǎn)品帶給中國客戶，并提供應用開發(fā)與培訓等多項服務；擁位于上海的中國創(chuàng)新中心，擁有有100多位專業(yè)研究人員和工程師及70多項專利。創(chuàng)新中心專注于針對垂直市場的產(chǎn)品研究和開發(fā)，結合中國市場的需求和國外先進技術，研發(fā)適合中國的技術和產(chǎn)品；我們擁有遍布全國的維修服務網(wǎng)點和特別成立的中國技術培訓團隊，在全國有超過2600名專業(yè)人員直接為客戶提供服務。我們致力于幫助客戶使世界更健康、更清潔、更安全。
欲了解更多信息，請登錄網(wǎng)站：www.thermofisher.com 全國服務熱線：021-61621892公司電子郵箱：sales.china@thermofisher.com
科學儀器
- GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility of this approach is suitable for small and large DNA constructs and includes both single and multiple inserts. The resulting products can be used for a variety of downstream applications including transformation, PCR and rolling circle amplification (RCA). The GeneArt Gibson Assembly HiFi Master Mix kit includes master mix, positive control, and water and accommodates the use of your own competent cells.
Features of the GeneArt Gibson Assembly HiFi Master Mix include:
? Simple—seamlessly assemble and clone up to six DNA fragments in a single reaction
? Efficient—high fidelity provides more correct clones than other methods
? Flexible—design guidelines allow assembly into any vector of your choice
? Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
? Trusted—over 4000 citations in the scientific literature highlighting great success
Seamless assembly of multiple fragments
GeneArt Gibson Assembly HiFi cloning is a simple, one-step process whereby up to six fragments are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After a 15–60 minute incubation, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones that are ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.
Robust method for maximum efficiency
Due to the covalently bound final product, the GeneArt Gibson Assembly HiFi method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, improving the success of finding the right clone, particularly for more challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.
Provides great versatility
The GeneArt Gibson Assembly HiFi method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.
Convenient formats
GeneArt Gibson Assembly HiFi kits are available in multiple formats, including:
? GeneArt Gibson Assembly HiFi Cloning Kit, chemically competent cells (with One Shot 0 chemically competent cells)
? GeneArt Gibson Assembly HiFi Cloning Kit, electrocompetent cells (with ElectroMAX DH10B electrocompetent cells)
? GeneArt Gibson Assembly HiFi Master Mix kits (cloning kit without the cells) (this page)
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