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A5185,0050Geno/mini DNA Isolation Spin-Kit(DNA提取試劑盒20ug)
參考價 | 面議 |
具體成交價以合同協(xié)議為準(zhǔn)
- 型號 A5185,0050
- 品牌
- 廠商性質(zhì) 代理商
- 所在地 上海市
更新時間:2017-07-13 20:39:57瀏覽次數(shù):859
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usage | for 50 or 250 isolations | |||
---|---|---|---|---|
HS-No. | 38220000 | |||
Storage | RT | |||
except for Porteinase K - Solution: +4°C | ||||
• Kit for the universal genomic DNA purification with spin minicolumns | ||||
• Applications: DNA suited for Southern blotting, PCR and Gene library cloning | ||||
• Advantage: One universal protocol for different types of samples | ||||
• Binding capacity of the column: 20 μg DNA | ||||
• Maximum sample volume: Blood (100 μl), cell culture cells (106 cells); bacteria (108 cells), tissue (15 mg) |
This kit is based on the property of DNA to adsorb to a silica surface in the presence of high concentrations of chaotropic salts. Cells or tissue are lysed with a special lysing solution, containing the chaotropic salt Total Lysis buffer AC, together with Proteinase K. In the next step the lysate containing the DNA is loaded onto spin columns. DNA is adsorbed on the surface of silica filter, while contaminants are passing through without adsorption. After washing, pure DNA is eluted in low salt buffer or water.