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北京西美杰科技有限公司
主營產(chǎn)品： 生物學(xué)檢測試劑盒,化學(xué)試劑,蛋白質(zhì)氧化損傷,抗原抗體 |
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A5253,0025DNA Swab Isolation Spin-Kit（咽試子DNA提取試劑盒）
| 參考價(jià) | 面議 |
具體成交價(jià)以合同協(xié)議為準(zhǔn)
- 型號 A5253,0025
- 品牌
- 廠商性質(zhì) 代理商
- 所在地 上海市
更新時(shí)間：2017-07-19 20:19:26瀏覽次數(shù)：780
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| HS-No. | 38220000 | |||
|---|---|---|---|---|
| Storage | RT | |||
| except for Proteinase K - Solution: +4°C | ||||
| • Kit for the DNA isolation from swabs with spin minicolumns | ||||
| • Advantage: approx. preparation time 30 minutes | ||||
| • Binding capacity of the column 10 μg DNA | ||||
| • sufficient for 25 and 100 isolations, respectively | ||||
This kit is based on the property of DNA to adsorb to silica surfaces in the presence of high concentrations of chaotropic salts. The lysis of cells from swabs is performed in the lysis solution containing chaotropic salts and nonionic detergents. The lysis process is supported by auxiliary, high affinity protease （Proteinase K）. Such reagents assure lysis and complete protein degradation. Subsequently the Iysate is loaded onto the spin minicolumn with dedicated silica membrane. The DNA binds to the silica membrane, while contaminants （fragmented proteins, salts. etc.） pass through the column freely. Residual contaminants are moved away by a wash step. Pure DNA is eluted with a low salt buffer or water and can be used in following experiments without further purification procedures （e.g. precipitation）.
