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[供應(yīng)]A1092,0100-Coomassie® Brilliant blue R-250 （C.I. 42660）考馬斯亮藍(lán)
A1092,0100-Coomassie® Brilliant blue R-250 （C.I. 42660）考馬斯亮藍(lán)
貨物所在地：
北京北京市
產(chǎn)地：
德國
更新時(shí)間：
2024-09-03 21:00:03
有效期：
2024年9月3日--2025年3月3日
已獲點(diǎn)擊：
238
【簡單介紹】Coomassie? Brilliant blue R-250 (C.I. 42660)，A1092,0100，100 g
【詳細(xì)說明】

Synonym Brilliant blue R, Xylenebrilliantcyanine 
Formula C45H44N3NaO7S2 
M 825.98 g/mol 
CAS-No. 6104-59-2 
HS-No. 32041200 
EC-No. 228-060-5 
Storage RT 
LGK 10 - 13 
WGK 2 
  ® registered trademark of Imperial Industries PLC 
Specification  
λmax. （buffer pH 7.0） 554 - 563 nm 
E 1 %,1 cm, λmax. >300 （pH 7.0） 

  （1）  Fazekas De St. Groth, S. et al. （1963） Biochim. Biophys. Acta 71, 377-391
Two new staining procedures for quantitative estimation of proteins on electrophoresis strips.
  （2）  Chrambach, A. et al. （1967） Anal. Biochem. 20, 150-154
A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.
  （3）  Neuhoff, V. et al. （1988） Electrophoresis 9, 255-262
Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie® Briliant Blue G-250 and R-250.
  （4）  Choi, J.-K. et al. （1996） Anal. Biochem. 236, 82-84
Modified Coomassie® Blue staining of proteins in polyacrylamide gels with Bismark brown.
  （5）  Tal, M. et al. （1985） J. Biol. Chem. 260, 9976-9980
Why does Coomassie® Brilliant Blue R Interact Differently with Different Porteins?

Coomassie® Brilliant Blue R-250 is one of the most commonly used stains for proteins, after their separation by polyacrylamide gel electrophoresis. The protein-dye complex has an absorption maximum at 549 nm, the dye without protein at 555 nm （in 0.01 M citrate buffer, pH 3）. The intensity in staining of proteins probably depends on the basicity of a protein （5）. Per positively charged amino acid approximay 1.5 - 3 molecules of Coomassie® will be bound. This variation complicate the exact protein determination with albumin as a standard, since this protein contains more basic amino acids than many other proteins （5）.
There do exist many protocols for sensitive staining procedures with Coomassie® （e. g. ref. 3, 4）. The sensitivity reaches a limit at 25 ng protein （4）. We recommend the following protocol:
I. Staining solution: 0.1 % Coomassie® Brilliant Blue R-250 （Prod.-No. A1092）
20 % methanol （or ethanol）
10 % acetic acid
The SDS gel （without 'stacking gel'） is stained for 1 hour at 60°C or for 2 hours at 50°C or over night at RT.
II. Destaining solution: 20 % methanol （or ethanol）
10 % acetic acid
Destain the gel for 3 - 4 hours at 50 - 60°C. Add some sponges.
Subsequently wash the gel for 15 minuts in water and dry under vacuum at 60°C for 2 - 3 hours.


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