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北京西美杰科技有限公司
主營產(chǎn)品： 生物學(xué)檢測試劑盒,化學(xué)試劑,蛋白質(zhì)氧化損傷,抗原抗體 |
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18210960361
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[供應(yīng)]A1360,1000 1 kg A1360,5000 5 kg A1360,9010 10 kg -Urea（尿素）
- 貨物所在地：
- 北京北京市
- 產(chǎn)地：
- 德國
- 更新時間：
- 2024-09-03 21:00:03
- 有效期：
- 2024年9月3日--2025年3月3日
- 已獲點擊：
- 368
【簡單介紹】Urea BioChemica
A1360,1000 1 kg
A1360,5000 5 kg
A1360,9010 10 kg
A1360,1000 1 kg
A1360,5000 5 kg
A1360,9010 10 kg
【詳細說明】
| Synonym | Carbamide | |||
|---|---|---|---|---|
| Solubility （20°C） | 1000 g/L （H2O） | |||
| Melting point | 132 - 135°C | |||
| Formula | CH4N2O | |||
| M | 60.06 g/mol | |||
| CAS-No. | 57-13-6 | |||
| HS-No. | 31021010 | |||
| EC-No. | 200-315-5 | |||
| Storage | RT | |||
| LGK | 10 - 13 | |||
| WGK | 1 | |||
| Specification | ||||
| Assay （from N） | min. 99 % | |||
| Heavy metals （as Pb） | max. 0.001 % | |||
| Water | max. 0.5 % | |||
| Chloride | max. 0.005 % | |||
| Sulfate | max. 0.005 % | |||
| Fe | max. 0.001 % | |||
| A （1 cm/8 M in H2O） | ||||
| 260 nm | max. 0.05 | |||
| 280 nm | max. 0.02 | |||
（1） Maniatis, T. & Efstratiadis, A （1980） Methods Enzymol. 65, 299-305
Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98 % formamide or 7 M urea.
（2） Soulié, S. et al. （1996） Anal. Biochem. 236, 363-364
Urea reduces the aggregation of membrane proteins during SDS-PAGE.
（3） Lippincott, J. & Apostol. I. （1999） Anal. Biochem. 267, 57-64
Carbamylation of cysteine in hemoglobin by urea.
Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98 % formamide or 7 M urea.
（2） Soulié, S. et al. （1996） Anal. Biochem. 236, 363-364
Urea reduces the aggregation of membrane proteins during SDS-PAGE.
（3） Lippincott, J. & Apostol. I. （1999） Anal. Biochem. 267, 57-64
Carbamylation of cysteine in hemoglobin by urea.
RNA or DNA denature at 7 M urea in polyacrylamide gels. Molecules with chain lengths larger than 150 - 200 nucleotides do not compley denature at room temperature in 7 M urea, so it is necessary to use polyacrylamide gels containing 98 % formamide to size larger molecules.
Separation of proteins according to their size and charge requires a concentration of 6-8 M urea in the probe and in the stacking and separation gel. At alkaline pH values urea decomposes and forms cyanate ions. These ions may react with the amino group and form stable carbamylated derivatives with a changed migration pattern during electrophoresis. Solutions containing urea should not be stored for longer periods, should not be heated and gels containing urea should prerun to remove cyanate ions before loading the gel.
Separation of proteins according to their size and charge requires a concentration of 6-8 M urea in the probe and in the stacking and separation gel. At alkaline pH values urea decomposes and forms cyanate ions. These ions may react with the amino group and form stable carbamylated derivatives with a changed migration pattern during electrophoresis. Solutions containing urea should not be stored for longer periods, should not be heated and gels containing urea should prerun to remove cyanate ions before loading the gel.
