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M CSF Mouse/重組小鼠巨噬細(xì)胞集落刺激因子
  • M CSF Mouse/重組小鼠巨噬細(xì)胞集落刺激因子
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貨物所在地:福建廈門市

更新時(shí)間:2024-11-24 21:00:06

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M CSF Mouse/重組小鼠巨噬細(xì)胞集落刺激因子
產(chǎn)品編號(hào):CYT-439
產(chǎn)品規(guī)格:2μg/10μg/1mg

Synonyms CSF-1, Lanimostim, MCSF, M-CSF.
Introduction Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Description Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD.
MCSF is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The protein (1mg/ml) was lyophilized with 5mM Tris, pH 8.0 + 100mM NaCl.
Solubility It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below
-18°C.
Please prevent freeze-thaw cycles.
Amino acid sequence MKEVSEHCSH MIGNGHLKVL LIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.
Purity Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found 0.91ng/ml .
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals

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