公司動(dòng)態(tài)
瓊脂糖凝膠4FF
閱讀:848 發(fā)布時(shí)間:2010-7-14
YY11664 | 瓊脂糖凝膠4FF | BR | 進(jìn)分 |
英文名稱:Sepharose 4FF
Exclusion limit:for globular proteins:~3×107 and for dextrans:~6×106
Mean particle size:90um
Bead size range:45~165um
Bead structure:highly cross-linked agarose,4% or 6%,respectively,spherical
Flow rate at 100kPa,25℃(XK 50/30 Column,15cm bed height):≥250cm/h
Recommended pH working range:2-12
PH stability,CIP:2-14
Chemical stability(40℃ for 7 days in):2M NaOH;70% ethanol;30% isopropanol;30% acetonitrile;1% SDS;2% Asepto;6 M guanidine-hydrochloride;8 M urea
性狀:乳白色液體。高度偶聯(lián)的瓊脂糖凝膠,大大加強(qiáng)了機(jī)械性能,快流速;經(jīng)去電核處理,非特異性吸附極低,樣品回收率高;*的化學(xué)穩(wěn)定性,可用多種促溶劑、有機(jī)溶劑操作和1-2M NaOH在位清洗
用途:巨大分子病毒、疫苗的分離
保存:2~8℃
Exclusion limit:for globular proteins:~3×107 and for dextrans:~6×106
Mean particle size:90um
Bead size range:45~165um
Bead structure:highly cross-linked agarose,4% or 6%,respectively,spherical
Flow rate at 100kPa,25℃(XK 50/30 Column,15cm bed height):≥250cm/h
Recommended pH working range:2-12
PH stability,CIP:2-14
Chemical stability(40℃ for 7 days in):2M NaOH;70% ethanol;30% isopropanol;30% acetonitrile;1% SDS;2% Asepto;6 M guanidine-hydrochloride;8 M urea
性狀:乳白色液體。高度偶聯(lián)的瓊脂糖凝膠,大大加強(qiáng)了機(jī)械性能,快流速;經(jīng)去電核處理,非特異性吸附極低,樣品回收率高;*的化學(xué)穩(wěn)定性,可用多種促溶劑、有機(jī)溶劑操作和1-2M NaOH在位清洗
用途:巨大分子病毒、疫苗的分離
保存:2~8℃
YY11665 | 丁基瓊脂糖凝膠4FF | BR | 進(jìn)分 |
英文名稱:Butyl Sepharose 4FF
CAS號(hào):157885-28-4
Base matrix:Sepharose 4 fast flow
Ligand:Butyl(C4H9)
Degree of substitution:approx 50 umol butyl/ml medium
Mean particle size:90um
Bead size range:45~165um
Bead structure:highly cross-linked 4% agarose,spherical
Max back pressure:0.1Mpa(1 bar 14psi)
Recomm linear flow rate:≤75% of packing flow rate
Linear flow rate at 25℃,XK 16/20,15cm bed height:≤300cm/h
PH stability:long term 3-13 and short term 2-14
Chemical stability(40℃ for 7 days in):1M NaOH;1mM HCL;70% ethanol;30% isopropanol;6 M guanidine-hydrochloride
Available capacity(mg/ml avained gel):26mg HAS
用途:適合純化疏水性更強(qiáng)的物質(zhì)。適合分離含脂族(aliphatic)配體的生物分子,可很好配合硫酸銨沉淀后樣品純化
保存:2~8℃
CAS號(hào):157885-28-4
Base matrix:Sepharose 4 fast flow
Ligand:Butyl(C4H9)
Degree of substitution:approx 50 umol butyl/ml medium
Mean particle size:90um
Bead size range:45~165um
Bead structure:highly cross-linked 4% agarose,spherical
Max back pressure:0.1Mpa(1 bar 14psi)
Recomm linear flow rate:≤75% of packing flow rate
Linear flow rate at 25℃,XK 16/20,15cm bed height:≤300cm/h
PH stability:long term 3-13 and short term 2-14
Chemical stability(40℃ for 7 days in):1M NaOH;1mM HCL;70% ethanol;30% isopropanol;6 M guanidine-hydrochloride
Available capacity(mg/ml avained gel):26mg HAS
用途:適合純化疏水性更強(qiáng)的物質(zhì)。適合分離含脂族(aliphatic)配體的生物分子,可很好配合硫酸銨沉淀后樣品純化
保存:2~8℃
YY11666 | 辛基瓊脂糖凝膠4FF | BR | 進(jìn)分 |
英文名稱:Octyl Sepharose 4FF
CAS號(hào):68652-09-5
Matrix:Highly cross linked agarose 4%
Type of ligand:Octyl:R-O-CH2-CH(OH)-CH2-O-(CH2)7-CH3
Bead form:R igid,spherical,macro porous
Mean particle size:90um diameter
Particle size distribution:45~165um
Degree of substitution:approx 5 umol octy/ml medium
PH stability:working range 3-13 and cleaning range 2-14
Autoclavable:20 min at 121℃
Linear flow rate at 25℃:≥150cm/h at 100kPa(1 bar, 14.5 psi);XK50/60,25cm bed height
Chemical stability:Stable in all commonly used aqueous buffers-1mM HCL;1M NaOH;(40℃,7 days) 30% isopropanol;70% ethanol; 6 M guanidine-hydrochloride;30% acetonitrile
用途:疏水性中等,適合各種蛋白的分離和純化
保存:2~8℃
CAS號(hào):68652-09-5
Matrix:Highly cross linked agarose 4%
Type of ligand:Octyl:R-O-CH2-CH(OH)-CH2-O-(CH2)7-CH3
Bead form:R igid,spherical,macro porous
Mean particle size:90um diameter
Particle size distribution:45~165um
Degree of substitution:approx 5 umol octy/ml medium
PH stability:working range 3-13 and cleaning range 2-14
Autoclavable:20 min at 121℃
Linear flow rate at 25℃:≥150cm/h at 100kPa(1 bar, 14.5 psi);XK50/60,25cm bed height
Chemical stability:Stable in all commonly used aqueous buffers-1mM HCL;1M NaOH;(40℃,7 days) 30% isopropanol;70% ethanol; 6 M guanidine-hydrochloride;30% acetonitrile
用途:疏水性中等,適合各種蛋白的分離和純化
保存:2~8℃
YY11667 | DNA純化瓊脂糖凝膠FF | BR | 源葉 |
英文名稱:PlasmidSelect DNA
特性:基團(tuán)脫落少,結(jié)合特異性強(qiáng)
基質(zhì):6%的交聯(lián)瓊脂糖凝膠
配基密度:20-40% umol/ml
吸附載量:≥1mg超螺旋DNA/ml
親和填料的顆粒大小:45~165um
zui大流速:300cm/h
PH范圍:3-10,在位清洗時(shí)PH范圍可達(dá)到2-11
用途:新型DNA純化的親和填料,它能特異地吸附超螺旋DNA,這樣就能容易得到純度超過(guò)90%的超螺旋DNA,是分離超螺旋DNA的*工具。適用高純超螺旋DNA的小批量和大規(guī)模制備
保存:2~8℃
特性:基團(tuán)脫落少,結(jié)合特異性強(qiáng)
基質(zhì):6%的交聯(lián)瓊脂糖凝膠
配基密度:20-40% umol/ml
吸附載量:≥1mg超螺旋DNA/ml
親和填料的顆粒大小:45~165um
zui大流速:300cm/h
PH范圍:3-10,在位清洗時(shí)PH范圍可達(dá)到2-11
用途:新型DNA純化的親和填料,它能特異地吸附超螺旋DNA,這樣就能容易得到純度超過(guò)90%的超螺旋DNA,是分離超螺旋DNA的*工具。適用高純超螺旋DNA的小批量和大規(guī)模制備
保存:2~8℃
YY11668 | DEAE瓊脂糖凝膠FF | BR | 進(jìn)分 |
英文名稱:DEAE Sepharose FF
CAS號(hào):57407-08-6
Type of exchanger:diethylaminoethyl weak anion
Total ionic capacity:0.11-0.16mmol/ml gel
Available capacity:Thyroglobulin(MW669000):3.1mg/ml;HAS(MW68000):110mg/ml;a-lactalbumin(MW14300):100mg/ml
Bead structure:Highly cross linked agarose 6%
Mean particle size:90um diameter
Bead size range:45~165um
Max flow rate(XK 16/20):25ml/min
Max linear flow rate:750cm/h
Max operating pressure:0.3Mpa(3 bar 42 psi)
pH working range:2-9
PH stability:long range 3-12 and short range 1-14
Chemical stability:All commonly used aqueous buffers, 1M NaOH;8M guanidine hydrochloride;70% ethanol(tested at 40℃ for 7 days)
Physical stability:Negligible volume variation due to changes in pH or ionic strength
用途:適用于各種帶負(fù)性電荷生物大分子的濃縮純化等
保存:2~8℃
CAS號(hào):57407-08-6
Type of exchanger:diethylaminoethyl weak anion
Total ionic capacity:0.11-0.16mmol/ml gel
Available capacity:Thyroglobulin(MW669000):3.1mg/ml;HAS(MW68000):110mg/ml;a-lactalbumin(MW14300):100mg/ml
Bead structure:Highly cross linked agarose 6%
Mean particle size:90um diameter
Bead size range:45~165um
Max flow rate(XK 16/20):25ml/min
Max linear flow rate:750cm/h
Max operating pressure:0.3Mpa(3 bar 42 psi)
pH working range:2-9
PH stability:long range 3-12 and short range 1-14
Chemical stability:All commonly used aqueous buffers, 1M NaOH;8M guanidine hydrochloride;70% ethanol(tested at 40℃ for 7 days)
Physical stability:Negligible volume variation due to changes in pH or ionic strength
用途:適用于各種帶負(fù)性電荷生物大分子的濃縮純化等
保存:2~8℃
YY11669 | Q瓊脂糖凝膠FF | BR | 進(jìn)分 |
英文名稱:Q Sepharose FF
Type of exchanger:quaternary amine;strong anion
Exclusion limit:4×106 (globular proteins)
Total ionic capacity:0.18-0.25mmol/ml gel
Available capacity:Thyroglobulin(MW669000):3mg/ml;HAS(MW68000):120mg/ml;x-lactalbumin(MW14300):110mg/ml
Bead structure:Highly cross linked agarose 6%
Mean particle size:90um diameter
Bead size range:45~165um
Max flow rate(XK 16/20):25ml/min
Flow rate:400-700cm/h
Max operating pressure:0.3Mpa(3 bar 42 psi)
pH working range:2-12
PH stability:long range 2-12 and short range 1-14
Chemical stability:All commonly used aqueous buffers, 1M NaOH;8M guanidine hydrochloride;70% ethanol(tested at 40℃ for 7 days)
Physical stability:Negligible volume variation due to changes in pH or ionic strength
用途:適用于各種帶負(fù)性電荷生物大分子的濃縮純化等
保存:2~8℃
Type of exchanger:quaternary amine;strong anion
Exclusion limit:4×106 (globular proteins)
Total ionic capacity:0.18-0.25mmol/ml gel
Available capacity:Thyroglobulin(MW669000):3mg/ml;HAS(MW68000):120mg/ml;x-lactalbumin(MW14300):110mg/ml
Bead structure:Highly cross linked agarose 6%
Mean particle size:90um diameter
Bead size range:45~165um
Max flow rate(XK 16/20):25ml/min
Flow rate:400-700cm/h
Max operating pressure:0.3Mpa(3 bar 42 psi)
pH working range:2-12
PH stability:long range 2-12 and short range 1-14
Chemical stability:All commonly used aqueous buffers, 1M NaOH;8M guanidine hydrochloride;70% ethanol(tested at 40℃ for 7 days)
Physical stability:Negligible volume variation due to changes in pH or ionic strength
用途:適用于各種帶負(fù)性電荷生物大分子的濃縮純化等
保存:2~8℃