CAS號(hào)9001-12-1膠原酶Ⅱ
貨號(hào)：C8150
規(guī)格：100mg/支
外觀（性狀） : 棕色或棕褐色結(jié)晶性粉末 
儲(chǔ)存條件 : -20℃ 
純度 : 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid 
別名 : 梭菌肽酶 A 
英文名稱 : CollagenaseⅡ 
單位 : 支

 膠原酶Ⅱ適用于肝臟，骨，甲狀腺，心臟和唾液腺組織細(xì)胞的分離。

膠原蛋白酶 來源于溶組織梭菌

Collagenase from Clostridium histolyticum

release of physiologically active rat epididymal adipocytes tested, Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Biochem/physiol Actions

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Caution

As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains * activity over 7 hours when held on ice.

Preparation Note

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Related Categories    3.4.x.x Peptidases, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Cell Dissociation,

Cell Dissociation and Cell Lysis, Cell Signaling Enzymes, Collagenases, Core Bioreagents, Crude Collagenase, Use-Tested, Cytoskeleton and Extracellular Matrix, Enzyme Class Index, Enzymes, Enzymes for Diagnostic Kit Manufacturing, Extracellular Matrix, Extracellular Matrix Peptides and Proteins, Histology, MMP Enzymes, Matrix Metalloproteinases Products, Other Apoptosis Related Reagents, Proteases, Proteases & Protein Sequencing, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Research Essentials, 生化試劑, 細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)和神經(jīng)科學(xué), 細(xì)胞生物學(xué), 酶、抑制劑和底物

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suitability       release of physiologically active rat epididymal adipocytes tested

Featured Industry       Diagnostic Assay Manufacturing

shipped in       wet ice

storage temp.       −20°C

CAS號(hào)9001-12-1膠原酶Ⅱ訂購說明：
1、絕大部分產(chǎn)品備有現(xiàn)貨，一般情況下都能訂貨當(dāng)日發(fā)貨。 
2、部分非常用產(chǎn)品，需提前1－2日預(yù)訂，海外期貨則需要提前3-6周預(yù)訂。 
3、部分產(chǎn)品價(jià)格會(huì)因貨期、批次等因素發(fā)生變化，若有變動(dòng)以訂貨當(dāng)日新價(jià)格為準(zhǔn)。 
4、每日訂單截止時(shí)間為16點(diǎn)整，部分城市可到17點(diǎn)整，因超過截止時(shí)間造成當(dāng)日不能發(fā)貨的將于次日安排發(fā)貨。
5、請辦理完貨款后，將收據(jù)、底單等連同收貨人的地址、姓名、等以傳真、、短信、等形式通知我們。

CAS號(hào)9001-12-1膠原酶Ⅱ運(yùn)輸說明：
1、極低溫產(chǎn)品：極低溫產(chǎn)品運(yùn)輸過程中加裝干冰運(yùn)輸。用干冰把產(chǎn)品包裹起來，再用泡沫盒密封，膠帶層層粘住泡沫盒，放入索萊寶的箱子，然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
2、低溫產(chǎn)品：低溫產(chǎn)品運(yùn)輸過程中加裝索萊寶冰袋運(yùn)輸。事先用冰袋把產(chǎn)品包裹起來，再使用泡沫盒密封，用膠帶嚴(yán)實(shí)密封泡沫盒，再放入索萊寶的箱子（保溫效果少可以持續(xù)一周），然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
3、常溫產(chǎn)品：常溫產(chǎn)品運(yùn)輸過程中無需加冰或者特殊包裝。產(chǎn)品由公司庫房人員快速配貨，準(zhǔn)確快速高效的快遞保證產(chǎn)品快速送達(dá)您的手中。