用途:用于快速檢測(cè)牛奶、奶粉等樣品中的三聚氰胺殘留。
原理: 本試劑盒采用間接競(jìng)爭(zhēng)ELISA 方法,在微孔條上預(yù)包被偶聯(lián)抗原,樣本中的殘留物三聚氰胺將和微孔條上預(yù)包被的偶聯(lián)抗原競(jìng)爭(zhēng)抗三聚氰胺抗體,加入酶標(biāo)記物后,用TMB 底物顯色,樣本吸光值與其所含殘留物三聚氰胺的含量成負(fù)相關(guān),與標(biāo)準(zhǔn)曲線(xiàn)比較即可得出相應(yīng)殘留物三聚氰胺的含量。
試劑盒特性:
u 試劑盒靈敏度:0.5ppb
u 孵育溫度: 25℃
u 孵育時(shí)間: 30min~15min
u 樣本檢測(cè)下限
牛奶··············································· 1 ppb
奶粉·············································· 10ppb
u 交叉反應(yīng)率
三聚氰胺········································· 100%
u 樣本回收率
牛奶······································· 90% ±25%
奶粉······································· 80% ±25%
試劑盒組成:
1 | 微量測(cè)試孔 | 每條8孔,一板12條 | |
2 | 標(biāo)準(zhǔn)液×6瓶 (1ml/瓶) | 0ppb | 0.5ppb |
1.5ppb | 4.5ppb | ||
13.5ppb | 40.5ppb | ||
3 | 酶標(biāo)記物 | 7ml | 紅色帽 |
4 | 抗體工作液 | 7ml | 藍(lán)色帽 |
5 | 底物A液 | 7ml | 白色帽 |
6 | 底物B液 | 7ml | 黑色帽 |
7 | 終止液 | 7ml | 黃色帽 |
8 | 20X濃縮洗滌液 | 40ml | 白色帽 |
樣本前處理及酶標(biāo)免疫分析程序:
詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
儲(chǔ)藏條件和保質(zhì)期:
1、儲(chǔ)藏條件:試劑盒于2~8℃保存,不要冷凍。
2、保質(zhì)期:該產(chǎn)品有效期為1 年,生產(chǎn)日期見(jiàn)包裝盒。
實(shí)驗(yàn)代做項(xiàng)目報(bào)價(jià)