Applied BioProbes 推出一系列熒光素和生物素標(biāo)記dUTP和UTP探針。這些探針可廣泛應(yīng)用于FISH, TUNEL, DNA microarray等應(yīng)用。我們的探針采用熒光亮度強(qiáng)，光穩(wěn)定性好的Andy Fluor染料標(biāo)記，并且在染料和dUTP及UTP之間設(shè)計(jì)一條特殊的X-linker以確保在酶的作用下更有效的插入DNA或RNA。

Figure 1. Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with Andy Fluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with Andy Fluor 488-X-dUTP (green) and Andy Fluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.

Figure 2. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with AndyFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).