Applied BioProbes 推出一系列核酸定量檢測(cè)試劑盒iQuant™ Nucleic Acid Quantitation Kits。這些試劑盒采用Applied BioProbes開發(fā)的新型核酸染料與DNA或RNA特異性結(jié)合產(chǎn)生熒光，從而實(shí)現(xiàn)精確而靈敏的熒光定量。與傳統(tǒng)用NanoDrop測(cè)吸收的方法相比，iQuant™ Nucleic Acid Quantitation Kits具有靈敏性高、線性范圍寬、定量更精確等優(yōu)點(diǎn)，并且與Life Technologies 的 Qubit® 熒光儀可以*兼容使用。

產(chǎn)品特點(diǎn)

◆靈敏度高：比傳統(tǒng)的NanoDrop高100倍的靈敏度；
◆線性范圍寬：比傳統(tǒng)的NanoDrop擁有更寬的線性區(qū)間；
◆特異性好：可分別實(shí)現(xiàn)對(duì)DNA或RNA定量；
◆操作簡(jiǎn)單：mix-and-read format;
◆適用性廣：可使用熒光酶標(biāo)儀，也可兼容熒光儀比如Qubit®，Quantus™。?

DNA quantitation of purified DNA samples is a standard nucleic acid research workflow, providing confidence for downstream applications such as Next Gen Sequence, PCR, Cloning, and DNA transfections (Figure 1).

Figure 1. Nucleic acid research workflow

In addition, Applied BioProbes' iQuant™ DNA Quantitation Kits provide higher sensitivity than absorbance-based quantitation methods, such as NanoDrop (Figure 2).

                             
Figure 2. Improved sensitivity with iQuant™ DNA Quantitation Kits

Further, iQuant™ DNA Quantitation Kits exhibit excellent selectivity for DNA, even in the presence of an equal mass of RNA (Figure 3).

Figure 3. Selectivity for dsDNA using iQuant™ HS dsDNA Quantitation Kit in the presence of an equal mass of RNA.

iQuant™ DNA Quantitation Kits show superior accuracy for detecting dsDNA compared with NanoDrop, especially at relatively low DNA concentrations (Figure 4).

Figure 4. Comparison of accuracy of DNA quantitation between the iQuant™ HS dsDNA Quantitation Kit and NanoDrop.

The iQuant™ DNA kits for DNA quantitation are simple to use. Just mix your DNA sample with iQuant™ dye solution, and perform the assays in either a multi-well plate reader, or in single tubes (Figure 5). The iQuant™ kits are compatible with the Qubit® fluorometer from Life Technologies and the Quantus™ fluorometer from Promega.

Figure 5. Assay workflow for iQuant™ DNA Quantitation Kits