Petaka三維微重力培養(yǎng)裝置氧氣濃度,
三維微重力培養(yǎng)裝置（Petaka™ G3 cell culture devices Microgravity 3D cell culture）

Petaka™ G3 cell culture devices / bioreactors can be used to grow aggregates of cells forming pseudo-tissu

Microgravity 3D cell culture

Petaka™ G3 cell culture devices / bioreactors can be used to grow aggregates of cells forming pseudo-tissues from any kind of differentiated cells or stem cells. These agregates , can be implanted into an organisms for regenerative research, cancer research and regenerative therapy experimentation

 

Differentiated cells and tumor cells forming 3D structures provide important experimental information about cell behavior in real tissues.

 

Microgravity 3D cultures can be obtained  in Petaka™ bioreactor when this is subjected to a controlled and adaptive rotation, keeping the cells in microgravity conditions that induces the formation of 3D cell clusters (pseudo-tissues).

 

Cystic 3D cell culture

 

This system is suitable to investigate intercellular interactions through soluble mediators, using the simple and unique bioreactor Petaka G3. Conceptually this bioreactor allows: 

1)     Growth of more than one cell type, separated by an inter-space full of media.
2)     Growth of several million cells, letting repeated cell sampling operations without interruption of the culture.
3)     Separate sampling of the different cell types included in the co-culture.
4)     Repeated media renewal and sampling operations without the interruption of the culture.

The biological product of this concept is the “Cystic 3D cell culture” that offers, in a single volume, at least three separate sheets of cells, with or without matrixes, growing with well defined borders, surrounding apocket (cyst) with 15 to 25 mL of shared media (see upper figure). The system is a cube like structure with 6 sides or 3 pairs of opposed walls facing each other. In this system the central cavity or cyst receives everything secreted or released by the surrounding cells including metabolites, extracellular organelles, exosomes, shedding microvesicles, apoptotic blebs and others. Cell shits are attached to the walls, uncoated or coated with specific matrix of single molecules such as Laminin, Vitronectin, Collagen, etc. or molecular mixtures such as Matrigel or others, all favoring cell attachment through integrins. One side of the device may be coated with a thick layer of semisolid gel allowing the formation of cell clusters and cell penetration evaluation (invasion assays).

Soluble secretions and particulate elements released by cells forming the walls circulate randomly through the cyst media, like in the blastocysts, ovary follicles and others. Those suspended elements become in contact with the other wall cells dragged by soft and slow convection streams, generated by controlled temperature gradients,.  Many physiologic reactions are controlled by soluble molecules and particulate elements, such as exosomes released from living cells 

The bottom side layer of cells is preferably influenced by particulate elements, it receives by gravity sedimentation all those elements having density over 1, released by the upper cells; this influence is even more intensive on all cells living on the side of the cyst when exposed to a multiplied g force vector, if the device is subject to a convenient centrifugation.

With an accurate control of hypoxia, close to physiologic DO (about 2 ppm), CO2 control and dehydration control (Petaka G3 conditions), these cultures could evolve for more than 2 weeks, allowing long term inter-cellular interactions. The cyst culture system (Petaka G3 device) allows for free-enzyme cell release of all the cells or samples of each cell type separay, therefore sampling each side independently for microscopy, FACS, PCR or biochemistry analysis. 

 

細胞培養(yǎng)新時代--PetakaG3*秀的細胞培養(yǎng)裝置！

PetakaG3^TM*秀的細胞培養(yǎng)裝置！

Celartia公司開發(fā)的PetakaG3^TM新型細胞培養(yǎng)裝置，利用可視化的全密封技術(shù)，降低了污染的風(fēng)險；*的氣體交換阻隔系統(tǒng)，使其不需要CO₂和濕度控制即可實現(xiàn)細胞健康生長。該培養(yǎng)裝置還優(yōu)化培養(yǎng)基/細胞產(chǎn)量比值和生長因子需求，并實現(xiàn)了在同一塊培養(yǎng)板裝置里細胞的培養(yǎng)和收集，操作簡便，功能多樣。

特點：

*  可使培養(yǎng)器內(nèi)的O₂濃度維持在狀態(tài)（不會供應(yīng)過量的O₂）

*  無須CO₂（可在CO₂培養(yǎng)箱內(nèi)培養(yǎng)）

*  無須加濕（幾乎不受外部濕度影響）

*  安全，不必擔心污染（無蓋、不開放、無泄漏）

*  可進行長時間的細胞觀察

*  堅固（沒有容易損壞的薄膜）

*  更適合于細胞長途安全運輸（無須冰凍、干冰運輸）

*  添加了的培養(yǎng)基和成長因子，約20ml～24ml培養(yǎng)基提供 150cm²成長面

*  提供約為體積15倍的細胞培養(yǎng)面

*  增殖的細胞可長期放置，并維持休眠狀態(tài)（只需放于室溫條件下）

Petaka培養(yǎng)板的應(yīng)用：

*  普通細胞的培養(yǎng)：使用zui少量的培養(yǎng)基，獲得更多的細胞

*  低氧細胞培養(yǎng)：無需特殊的培養(yǎng)箱或手套箱，即可實現(xiàn)低氧細胞培養(yǎng)

*  細胞長距離運輸：安全、高效的長距離細胞運輸?shù)?選擇

*  細胞的休眠與復(fù)蘇：對細胞生物學(xué)、毒性學(xué)無影響

*  細胞存儲：使得細胞存儲變得很便利

*  細胞庫管理：細胞生長，管理和運輸變得簡單

貨號	品名	包裝	品牌	用途
PET025	petaka G3 Cell Culture Device Pack LOT	25塊	Celartia	動物細胞培養(yǎng)
PET300	petaka G3 Cell Culture Device Box（12*25 Pieces）LOT	12*25塊	Celartia	動物細胞培養(yǎng)
PET120	petaka G3 Cell Culture Device Box（48*25 Pieces）LOT	48*25塊	Celartia	動物細胞培養(yǎng)
PET025H	petaka G3 Cell Culture Device Pack HOT	25塊	Celartia	昆蟲細胞培養(yǎng)
PET300H	petaka G3 Cell Culture Device Box（12*25 Pieces） HOT	12*25塊	Celartia	昆蟲細胞培養(yǎng)
PET120H	petaka G3 Cell Culture Device Box（48*25 Pieces） HOT	48*25塊	Celartia	昆蟲細胞培養(yǎng)
PET025F	petaka G3 flat Cell Culture Device Pack	25塊	Celartia	懸浮細胞培養(yǎng)
PET300F	petaka G3 flat Cell Culture Device Box（12*25 Pieces）	12*25塊	Celartia	懸浮細胞培養(yǎng)
PET120F	petaka G3 flat Cell Culture Device Box（48*25 Pieces）	48*25塊	Celartia	懸浮細胞培養(yǎng)
PET025ET	petaka EF LOT Cell Culture Device Pack	25塊	Celartia	*回收細胞
PET300ET	petaka EF LOT Cell Culture Device Box（12*25 Pieces）	12*25塊	Celartia	*回收細胞
PET120ET	petaka EF LOT Cell Culture Device Box（48*25 Pieces）	48*25塊	Celartia	*回收細胞
PET025ETH	petaka EF HOT Cell Culture Device Pack	25塊	Celartia	*回收細胞
PET300ETH	petaka EF HOT Cell Culture Device Box（12*25 Pieces）	12*25塊	Celartia	*回收細胞
PET120ETH	petaka EF HOT Cell Culture Device Box（48*25 Pieces）	48*25塊	Celartia	*回收細胞
PETST6	petaka G3 Stands STANDS Box紅 藍 橙 紫 綠 黃6色	12快	Celartia	細胞培養(yǎng)架子
TIP120	petaka G3 Tip Blunt 18 Guage Box 頓口針頭	120支	Celartia	進樣用
PET010	petaka Transporter Envelopes 運輸信封	10只	Celartia	細胞運輸用
TRAIN02	petaka G3 LOT Training kit contains 50 petakas 6 stands & 120 tips	kit	Celartia	初次實驗人員套裝
TRAIN01	petaka G3 HOT Training kit contains 50 petakas 6 stands & 120 tips	kit	Celartia	初次實驗人員套裝
TRAIN01ET	petaka ET LOT Training kit 300x petakaET 1x Clearcell 360x tips	kit	Celartia	初次實驗人員套裝
TRAIN02ET	petaka ET HOT Training kit 300x petakaET 1x Clearcell 360x tips	kit	Celartia	初次實驗人員套裝
CCLER1	Clearcell magnetic field generator	臺	Celartia	*回收細胞
CENT21	DIGICEN21 CENTRIFUGE 離心機	臺	Celartia	細胞離心