MaxOne細(xì)胞電刺激培養(yǎng)成像記錄電生理系統(tǒng)--提供解決方案以推進(jìn)基礎(chǔ)神經(jīng)科學(xué)研究并加速臨床前藥物發(fā)現(xiàn)。 我們研制的MaxOne是體外記錄和刺激原電細(xì)胞（腦，視網(wǎng)膜和心臟細(xì)胞）的Z強(qiáng)大的電生理學(xué)平臺(tái)。 MaxOne是一種基于CMOS的高密度微電極陣列，具有26,400個(gè)電極，1024個(gè)低噪聲讀出通道和32個(gè)刺激通道。 MaxOne允許提取有助于表型篩選和藥物毒性/功效測(cè)試單細(xì)胞和網(wǎng)絡(luò)參數(shù)。

MaxOne細(xì)胞電刺激培養(yǎng)成像記錄電生理系統(tǒng)

MaxOne是記錄和刺激體外細(xì)胞的zui強(qiáng)大的電生理平臺(tái)。 MaxOne是單孔格式的高密度微電極陣列（HD-MEA）系統(tǒng)。
MaxOne是一款基于CMOS的高密度微電極陣列（HD-MEA）系統(tǒng)。
這使得緊湊而又功能強(qiáng)大的放大器，濾波器和數(shù)字化儀能夠集成到MEA中，并且與待研究的細(xì)胞緊密相連。

?高分辨率，無(wú)標(biāo)記細(xì)胞活動(dòng)成像
?亞細(xì)胞和網(wǎng)絡(luò)活動(dòng)的無(wú)標(biāo)記檢測(cè)
?多種選擇性電刺激
?全面的數(shù)據(jù)分析工具

 

從原發(fā)組織中收集細(xì)胞，如大腦，視網(wǎng)膜或心臟。

準(zhǔn)備實(shí)驗(yàn)時(shí)，開(kāi)始在MaxWell Biosystems的MEA芯片上培養(yǎng)細(xì)胞。

開(kāi)始您的實(shí)驗(yàn)：在MaxLab Live軟件的幫助下，跟蹤結(jié)果并在計(jì)算機(jī)上分析數(shù)據(jù)。

使用結(jié)果來(lái)指導(dǎo)進(jìn)一步的實(shí)驗(yàn)，獲得科學(xué)見(jiàn)解并發(fā)布數(shù)據(jù)。

Multiscale recording and analysis —

1. High-resolution, label-free cell activity imaging

Analyze subcellular features, such as full axonal arbors in single neurons.

Electrical signals tracked along neurites enable to investigate novel parameters. High-resolution tracking of axonal action potential propagation allows for investigating changes in axonal conduction velocity.

 

2. Whole sample activity map

Localize active cells on the MEA during experiments.

The morphological structure of a primary cortical cell culture was imaged using an upright microscope with 10x objective. MAP2 staining was used to visualize the neurons. The optical image closely matches the electrical image obtained using MaxOne. The electrical image provides the location of the cells, as well as information on the activity of the cells, such as spike rate and amplitude.

 

 

3. Smart population recording

Identify defined cells for long term recording and further analyses.

Different combinations of parameters can be used to automatically select a defined set of cells to be recorded, such as spike rate, amplitude, etc. The raster plot shows the dynamics of the network activity using 1,024 active electrode sites. Burst features can be investigated in detail (one burst zoomed in).