活性氧檢測試劑盒(Reactive Oxygen Species Assay Kit)（CAT# 50101ES01）是一種基于熒光染料DCFH-DA (2,7-Dichlorodi -hydrofluorescein diacetate)的熒光強(qiáng)度變化，定量檢測細(xì)胞內(nèi)活性氧水平方法。

DCFH-DA本身沒有熒光，可以自由穿過細(xì)胞膜，進(jìn)入細(xì)胞內(nèi)后，可以被細(xì)胞內(nèi)的酯酶水解生成DCFH。而DCFH不能透過細(xì)胞膜，從而使探針很容易被標(biāo)記聚集在細(xì)胞內(nèi)。細(xì)胞內(nèi)的活性氧能夠氧化無熒光的DCFH生成有熒光的DCF。DCF綠色熒光強(qiáng)度與細(xì)胞內(nèi)活性氧水平成正比，檢測DCF的熒光就可以知道細(xì)胞內(nèi)活性氧水平。

在激發(fā)波長488 nm，發(fā)射波長525 nm條件下，使用熒光顯微鏡、激光共聚焦顯微鏡、熒光分光光度計(jì)、熒光酶標(biāo)儀、流式細(xì)胞儀等檢測DCF熒光，從而測定細(xì)胞內(nèi)活性氧水平。

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