【文獻標題】Bacteroides-derived isovaleric acid enhances mucosal immunity by facilitating intestinal IgA response in broilers

【作者】Xinkai Wang, Yifan Huet.al

【作者單位】 華中農(nóng)業(yè)大學（Huazhong Agricultural University）

【文獻中引用產(chǎn)品】

雞免疫球蛋白A(IgA)ELISA試劑盒

雞免疫球蛋白M(IgM)ELISA試劑盒

【關鍵詞】Bacteroides, Chicken, IgA, Intestinal health, Isovaleric acid, Macrophage

【DOI】doi.org/10.1186/s40104-022-00807-y

【影響因子(IF)】6.17

【出版期刊】《Journal of Animal Science and Biotechnology》

【產(chǎn)品原文引用】Intestinal contents or tissues samples were soaked in PBS (100μL to 10mg sample) and homogenized using homogenizer, then centrifuged at 12,000 ×g for 10min to remove small particles, and the supernatant was collected. Free IgA and IgM concentration were detected using chicken IgA, and IgM enzyme-linked immunosorbent assay (ELISA) quantitation Set (HUDING Biology, 

Shanghai, China).

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