重慶市華雅干細(xì)胞技術(shù)有限公司 |
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origen 現(xiàn)貨熱賣,歡迎咨詢?。?!
閱讀:1422發(fā)布時(shí)間:2020-11-10
ORIGEN 授權(quán)代理,種類齊全,現(xiàn)貨供應(yīng),現(xiàn)雙十一*,*銷售,歡迎咨詢!!!
背景知識(shí):
細(xì)胞凍存是細(xì)胞保存的主要方法之一。利用凍存技術(shù)將細(xì)胞置于-196℃液氮中低溫保存,可以使細(xì)胞暫時(shí)脫離生長(zhǎng)狀態(tài)而將其細(xì)胞特性保存起來(lái),這樣在需要的時(shí)候再?gòu)?fù)蘇細(xì)胞用于實(shí)驗(yàn)。而且適度地保存一定量的細(xì)胞,可以防止因正在培養(yǎng)的細(xì)胞被污染或其他意外事件而使細(xì)胞丟種,起到了細(xì)胞保種的作用。除此之外,還可以利用細(xì)胞凍存的形式來(lái)購(gòu)買、寄贈(zèng)、交換和運(yùn)送某些細(xì)胞。 細(xì)胞凍存時(shí)向培養(yǎng)基中加入保護(hù)劑--終濃度5%.15%的甘油或二甲基亞砜(DMSO),可使溶液冰點(diǎn)降低,加之在緩慢凍結(jié)條件下,細(xì)胞內(nèi)水分透出,減少了冰晶形成,從而避免細(xì)胞損傷。 采用"慢凍快融"的方法能較好地保證細(xì)胞存活。標(biāo)準(zhǔn)冷凍速度開(kāi)始為-1 到 -2℃/min,當(dāng)溫度低于-25℃時(shí)可加速,到-80℃之后可直接投入液氮內(nèi)(-196℃)。
二甲基亞砜(DMSO)是一種含硫有機(jī)化合物,常溫下為無(wú)色無(wú)味的透明液體,是一種吸濕性的可燃液體,具有高極性、高沸點(diǎn)、熱穩(wěn)定性好、非質(zhì)子、與水混溶的特性,能溶于乙醇、丙醇、苯等大多數(shù)有機(jī)物,被譽(yù)為“萬(wàn)能溶劑”。二甲基亞砜是一種重要的滲透性細(xì)胞保護(hù)劑。在深低溫(零下200度)保存細(xì)胞時(shí)凍存過(guò)程中,為防止細(xì)胞內(nèi)液冰晶形成、滲透壓改變、細(xì)胞結(jié)構(gòu)紊亂等導(dǎo)致的損傷,有必要使用含有DMSO冷凍保護(hù)劑。DMSO能夠快速穿透細(xì)胞膜進(jìn)入細(xì)胞中,降低冰點(diǎn),延緩凍存過(guò)程,同時(shí)提高細(xì)胞內(nèi)離子濃度,減少細(xì)胞內(nèi)冰晶的形成,從而減少細(xì)胞損傷。深低溫時(shí)二甲基亞砜的細(xì)胞毒性受到抑制。復(fù)蘇時(shí)動(dòng)作要快,盡快洗掉二甲基亞砜,否則會(huì)造成對(duì)細(xì)胞嚴(yán)重的毒性。二甲基亞砜(DMSO)是目前的細(xì)胞凍存保護(hù)劑,但也是一種細(xì)胞毒性很大的化學(xué)試劑。研究結(jié)果表明,培養(yǎng)液中DMSO濃度為10%時(shí),細(xì)胞生長(zhǎng)抑制率近100%;1%濃度時(shí)抑制率為35%,即使是0.04%的濃度,DMSO對(duì)細(xì)胞的生長(zhǎng)也有不利的影響。
右旋糖酐系蔗糖是經(jīng)腸膜狀明串珠菌-1226(Leuconostoc mesenteroides)發(fā)酵合成的一種高分子葡萄糖聚合物,右旋糖酐-40(Dextran-40)別稱低分子右旋糖酐,右旋糖酐40的重均分子量(Mw)應(yīng)為32000~42000。右旋糖酐-40可以維持滲透壓和 PiH , 提供適宜細(xì)胞存活的介質(zhì)環(huán)境。
OriGen公司研發(fā)的二甲亞砜(DMSO)在高級(jí)別DMSO的精制領(lǐng)域中處于地位,其細(xì)胞凍存級(jí)產(chǎn)品在市場(chǎng)上擁有較高的占有率,經(jīng)過(guò)十多年的客戶檢驗(yàn)與使用,質(zhì)量過(guò)硬。
ORIGEN 授權(quán)代理,種類齊全,現(xiàn)貨供應(yīng),現(xiàn)雙十一*,*銷售,歡迎咨詢?。?
*
凍存袋 | 貨號(hào) | 名稱 | 規(guī)格 |
OriGen | CS25F | Freezing Bags,vol-max,10-25mL凍存袋 | 24個(gè)/箱 |
OriGen | CS25N | Freezing Bags,vol-max,10-25mL凍存袋 | 24個(gè)/箱 |
OriGen | CS50 | Freezing Bags,vol-max,10-30mL凍存袋 | 24個(gè)/箱 |
OriGen | CS50N | Freezing Bags,vol-max,10-30mL凍存袋,N型管路 | |
OriGen | CS250 | Freezing Bags,vol-max,30-70mL凍存袋 | 24個(gè)/箱 |
OriGen | CS250S | Freezing Bags,vol-max,30-70mL凍存袋 | 12個(gè)/箱 |
OriGen | CS250N | Freezing Bags,vol-max,30-70mL凍存袋,N型管路 | 24個(gè)/箱 |
OriGen | CS500 | Freezing Bags,vol-max,55-100mL凍存袋 | 24個(gè)/箱 |
OriGen | CS750 | Freezing Bags,vol-max,80-190mL凍存袋 | 24個(gè)/箱 |
OriGen | CS1000N | Freezing Bags,vol-max,120-275mL凍存袋,N型管路 | 24個(gè)/箱 |
OriGen | CS2000N | Freezing Bags,vol-max,180-450mL凍存袋 | 24個(gè)/箱 |
OriGen | CS2000NP (廠家停產(chǎn)) | Freezing Bags,vol-max,180-450mL凍存袋 | 12個(gè)/箱 |
OriGen | CS30M9 | 5腔室Cryostore Multi-Chamber Bag | 12個(gè)/箱 |
OriGen | CS24M9 | 4腔室Cryostore Multi-Chamber Bag | 12個(gè)/箱 |
培養(yǎng)袋 | 貨號(hào) | 名稱 | 規(guī)格 |
OriGen | PL07-2G | PermaLife Cell Culture Bags | 1個(gè)/包 |
OriGen | PL30-2G | PermaLife Cell Culture Bags | 1個(gè)/包 |
OriGen | PL70-2G | PermaLife Cell Culture Bags | 1個(gè)/包 |
OriGen | PL120-2G | PermaLife Cell Culture Bags | 1個(gè)/包 |
OriGen | PL240-2G | PermaLife Cell Culture Bags | 1個(gè)/包 |
OriGen | PL240K | PermaLife Cell Culture Bags,定制 | 1個(gè)/包 |
OriGen | PL325K | PermaLife Cell Culture Bags,定制 | 1個(gè)/包 |
OriGen | PL325-2G | PermaLife Cell Culture Bags | 1個(gè)/包 |
OriGen | PL500P-2G | PermaLife Cell Culture Bags(50個(gè)起訂) | 1個(gè)/包 |
OriGen | PL750-2G | PermaLife Cell Culture Bags | 1個(gè)/包 |
OriGen | PL1000P-2G | PermaLife Cell Culture Bags | 1個(gè)/包 |
OriGen | PL2000P2-2G | PermaLife Cell Culture Bags(50個(gè)起訂) | 1個(gè)/包 |
凍存液DMSO | 貨號(hào) | 名稱 | 規(guī)格 |
OriGen | CP-10 | Vial, 10ml CryoPur : 100% DMSO | 12瓶/盒 |
OriGen | CP-50 | Vial, 50ml CryoPur : 100% DMSO | 6瓶/盒 |
OriGen | CP-70 | Vial, 70ml CryoPur : 100% DMSO | 6瓶/盒 |
OriGen | CP-70-Bag | Vial, 70ml CryoPur : 100% DMSO | 70ml/瓶 |
OriGen | CP-100 | Vial, 100ml CryoPur : 100% DMSO | 6瓶/盒 |
OriGen | SP-10 | 10ml in 20ml syringe with stopcock and SCD tubing | 1 unit |
OriGen | SP-20 | 20ml in 20ml syringe with stopcock and SCD tubing | 1 unit |
OriGen | SP-30 | 30ml in 20ml syringe with stopcock and SCD tubing | 1 unit |
OriGen | SP-50 | 50ml in 20ml syringe with stopcock and SCD tubing | 1 unit |
混合液 | 貨號(hào) | 名稱 | 規(guī)格 |
OriGen | CD-07 | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection | |
OriGen | CD-35 | CryoPur-D™ : 55% DMSO, 5% Dextran-40w/v, remainder water for injection | |
OriGen | CD-50 | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection | 6瓶/盒 |
OriGen | CD-50-Bag | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection | 50ml/瓶 |
OriGen | CD-100-Bag | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection | 100ml/瓶 |
OriGen | SD-07 | CryoPur-D™ : 55% DMSO, 5% Dextran-40w/v, remainder water for injection,帶注射器 | |
OriGen | SD-10 | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection,帶注射器 | |
OriGen | SDS-05A-B | 5ml 55% DMSO, 5% Dextran 40 w/v, remainder 0.9% saline + 2ml air in 20ml syringe with stopcock and SCD tubing | |
OriGen | SDS-15A | 15ml 55% DMSO, 5% Dextran 40 w/v, remainder 0.9% saline in 20ml syringe with stopcock and SCD tubing | |
外袋 | 貨號(hào) | 名稱 | 規(guī)格 |
OriGen | OW0419 | 10個(gè)/包 | |
OriGen | OW0909 | O-Wrap Overwrap Bags 外袋(適合CS25) | 10個(gè)/包 |
OriGen | OW0918-S | O-Wrap Overwrap Bags 外袋(適合CS25,CS50,PL07) | 10個(gè)/包 |
OriGen | OW0918* | O-Wrap Overwrap Bags 外袋(適合CS25,CS50,PL07) | 10個(gè)/包 |
OriGen | OW0918-100S | O-Wrap Overwrap Bags 外袋(適合CS25,CS50,PL07) | 100個(gè)/箱 |
OriGen | OW1430 | O-Wrap Overwrap Bags 外袋(適合CS250,CS750,PL30,PL240) | 10個(gè)/包 |
OriGen | OW1936 | O-Wrap Overwrap Bags 外袋(適合CS1000) | 10個(gè)/包 |
OriGen | OW1950 | O-Wrap Overwrap Bags 外袋 | 10個(gè)/包 |
OriGen | OW2436 | O-Wrap Overwrap Bags 外袋(適合CS2000N) | 10個(gè)/包 |
*備注:OW0918*是由100個(gè)每箱的大包裝分裝成10個(gè)每包的小包裝 | |||
熱合儀 | 貨號(hào) | 名稱 | 規(guī)格 |
OriGen | CR6 | Biosealer CR 6 熱合儀 | 1臺(tái)/箱 |
OriGen | SZ1823 | SteriZip Overpouch(CS25, CS50 ) | 10個(gè)/包 |
OriGen | SZ2533 | SteriZip Overpouch(CS250, CS500, CS750 ) | 10個(gè)/包 |
OriGen | SZ3038 | SteriZip Overpouch(CS1000, CS2000) | 10個(gè)/包 |
OriGen | SZ2533-2M | SteriZip Overpouch,2.0毫米 | |
OriGen | SZ2533P-1 | SteriZip Overpouch,獨(dú)立包裝 | 50個(gè)/包 |
OriGen | SZ2533P-3 | SteriZip Overpouch,3個(gè)/小包 | 45個(gè)/包 |
配件 | 貨號(hào) | 名稱 | 規(guī)格 |
OriGen | S-F10 | One spike to one female luer with 10cm of tubing and pinch clamp, 100% DMSO Resistant | 50個(gè)/箱 |
OriGen | RF-T15 | 6ml syringe on a female luer with 15cm sealed SCD tubing | 25個(gè)/箱 |
OriGen | 15-RF60-T | 60ml syringe on a female luer with 15cm sealed SCD tubing | 25個(gè)/箱 |
OriGen | 15-RF12-T | 12ml syringe on a female luer with 15cm sealed SCD tubing | 25個(gè)/箱 |
OriGen | 15-RF20-T | 20ml syringe on a female luer with 15cm sealed SCD tubing | 25個(gè)/箱 |
OriGen | R3F-T15 | 3ml syringe on a female luer with 15cm sealed SCD tubing | 25個(gè)/箱 |
OriGen | 2S-P20 | Two spikes to one spike port with 20cm SCD tubing and pinch clamps | 20個(gè)/箱 |
OriGen | S-38B12 | One spike to one 3/8″ barb with 12cm DMSO resistant tubing, 100% DMSO Resistant | 25個(gè)/箱 |
OriGen | S-FFP12 | One spike to one spike port and two female luers with 12cm SCD tubing and pinch clamps | 20個(gè)/箱 |
OriGen | 4S-4M60 | Four spikes to four male luers with 60cm SCD tubing, roller and pinch clamps | |
OriGen | VSV | Vial spike with needle-free valve | 10個(gè)/包 |
OriGen | S-M50 | One spike to male luer with 50cm SCD tubing and pinch clamp | 50個(gè)/箱 |
OriGen | S-F50 | One spike to one female luer with 50cm SCD tubing and pinch clamp | 50個(gè)/箱 |
OriGen | S-S50 | One spike to one spike with 50cm of SCD tubing | 12個(gè)/箱 |
OriGen | CC2 | Cell Connect One spike and male luer to a 3-way stopcock, 60ml syringe and needle-free valve to four male luers with 70cm SCD tubing and pinch clamps | 12個(gè)/箱 |
OriGen | 12-F-M | One female luer to one male luer with 12cm tubing, 100% DMSO resistant | 50個(gè)/箱 |
OriGen | CBS | One spike with needle-free valve, 100% DMSO Resistant | |
淀粉 | 貨號(hào) | 名稱 | 規(guī)格 |
B.Braun | L6511 | 6%Hetastarch in 0.9%Sodium Chloride羥乙基淀粉氯化鈉 | 500ml/袋 |
B.Braun | L6511 | 6%Hetastarch in 0.9%Sodium Chloride羥乙基淀粉氯化鈉 | 12*500ml/箱 |
Hospira | 0409-7419-14-bag | 10% LMD in 0.9%Chlorde Injection右旋糖酐 | 500ml/袋 |
袋子 | 貨號(hào) | 名稱 | 規(guī)格 |
OriGen | EV120+F-M-12 | EVO Bag, 70-120ml Fill Volume, with luer connections and needle free valve with separate female to male tube set with 12cm of tubing | 20套/箱 |
OriGen | EV1000N | EVO Bag,100 -500ml Fill Volume, with one male luer with cap, two female luers with caps, and a needle-free injection port | 12個(gè)/箱 |
OriGen | EV3000N | EVO Bag, 500-2000ml Fill Volume, with one male luer with cap, two female luers with caps, and a needle-free injection port | 12個(gè)/箱 |
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