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15021010459

干細(xì)胞無(wú)血清培養(yǎng)基

干細(xì)胞因子及添加物

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相關(guān)檢測(cè)試劑

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細(xì)胞凍存

細(xì)胞重編程

蛋白質(zhì)/多肽

抗體/抗原

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origen 現(xiàn)貨熱賣,歡迎咨詢?。?!

閱讀:1422發(fā)布時(shí)間:2020-11-10

 ORIGEN 授權(quán)代理,種類齊全,現(xiàn)貨供應(yīng),現(xiàn)雙十一*,*銷售,歡迎咨詢!!!

 背景知識(shí):
細(xì)胞凍存是細(xì)胞保存的主要方法之一。利用凍存技術(shù)將細(xì)胞置于-196℃液氮中低溫保存,可以使細(xì)胞暫時(shí)脫離生長(zhǎng)狀態(tài)而將其細(xì)胞特性保存起來(lái),這樣在需要的時(shí)候再?gòu)?fù)蘇細(xì)胞用于實(shí)驗(yàn)。而且適度地保存一定量的細(xì)胞,可以防止因正在培養(yǎng)的細(xì)胞被污染或其他意外事件而使細(xì)胞丟種,起到了細(xì)胞保種的作用。除此之外,還可以利用細(xì)胞凍存的形式來(lái)購(gòu)買、寄贈(zèng)、交換和運(yùn)送某些細(xì)胞。 細(xì)胞凍存時(shí)向培養(yǎng)基中加入保護(hù)劑--終濃度5%.15%的甘油或二甲基亞砜(DMSO),可使溶液冰點(diǎn)降低,加之在緩慢凍結(jié)條件下,細(xì)胞內(nèi)水分透出,減少了冰晶形成,從而避免細(xì)胞損傷。 采用"慢凍快融"的方法能較好地保證細(xì)胞存活。標(biāo)準(zhǔn)冷凍速度開(kāi)始為-1 到 -2℃/min,當(dāng)溫度低于-25℃時(shí)可加速,到-80℃之后可直接投入液氮內(nèi)(-196℃)。
二甲基亞砜(DMSO)是一種含硫有機(jī)化合物,常溫下為無(wú)色無(wú)味的透明液體,是一種吸濕性的可燃液體,具有高極性、高沸點(diǎn)、熱穩(wěn)定性好、非質(zhì)子、與水混溶的特性,能溶于乙醇、丙醇、苯等大多數(shù)有機(jī)物,被譽(yù)為“萬(wàn)能溶劑”。二甲基亞砜是一種重要的滲透性細(xì)胞保護(hù)劑。在深低溫(零下200度)保存細(xì)胞時(shí)凍存過(guò)程中,為防止細(xì)胞內(nèi)液冰晶形成、滲透壓改變、細(xì)胞結(jié)構(gòu)紊亂等導(dǎo)致的損傷,有必要使用含有DMSO冷凍保護(hù)劑。DMSO能夠快速穿透細(xì)胞膜進(jìn)入細(xì)胞中,降低冰點(diǎn),延緩凍存過(guò)程,同時(shí)提高細(xì)胞內(nèi)離子濃度,減少細(xì)胞內(nèi)冰晶的形成,從而減少細(xì)胞損傷。深低溫時(shí)二甲基亞砜的細(xì)胞毒性受到抑制。復(fù)蘇時(shí)動(dòng)作要快,盡快洗掉二甲基亞砜,否則會(huì)造成對(duì)細(xì)胞嚴(yán)重的毒性。二甲基亞砜(DMSO)是目前的細(xì)胞凍存保護(hù)劑,但也是一種細(xì)胞毒性很大的化學(xué)試劑。研究結(jié)果表明,培養(yǎng)液中DMSO濃度為10%時(shí),細(xì)胞生長(zhǎng)抑制率近100%;1%濃度時(shí)抑制率為35%,即使是0.04%的濃度,DMSO對(duì)細(xì)胞的生長(zhǎng)也有不利的影響。
右旋糖酐系蔗糖是經(jīng)腸膜狀明串珠菌-1226(Leuconostoc mesenteroides)發(fā)酵合成的一種高分子葡萄糖聚合物,右旋糖酐-40(Dextran-40)別稱低分子右旋糖酐,右旋糖酐40的重均分子量(Mw)應(yīng)為32000~42000。右旋糖酐-40可以維持滲透壓和 PiH , 提供適宜細(xì)胞存活的介質(zhì)環(huán)境。
OriGen公司研發(fā)的二甲亞砜(DMSO)在高級(jí)別DMSO的精制領(lǐng)域中處于地位,其細(xì)胞凍存級(jí)產(chǎn)品在市場(chǎng)上擁有較高的占有率,經(jīng)過(guò)十多年的客戶檢驗(yàn)與使用,質(zhì)量過(guò)硬。

  ORIGEN 授權(quán)代理,種類齊全,現(xiàn)貨供應(yīng),現(xiàn)雙十一*,*銷售,歡迎咨詢?。?

*

凍存袋

貨號(hào)名稱規(guī)格
OriGenCS25FFreezing Bags,vol-max,10-25mL凍存袋24個(gè)/箱
OriGenCS25NFreezing Bags,vol-max,10-25mL凍存袋24個(gè)/箱
OriGenCS50Freezing Bags,vol-max,10-30mL凍存袋24個(gè)/箱
OriGenCS50NFreezing Bags,vol-max,10-30mL凍存袋,N型管路 
OriGenCS250Freezing Bags,vol-max,30-70mL凍存袋24個(gè)/箱
OriGenCS250SFreezing Bags,vol-max,30-70mL凍存袋12個(gè)/箱
OriGenCS250NFreezing Bags,vol-max,30-70mL凍存袋,N型管路24個(gè)/箱
OriGenCS500Freezing Bags,vol-max,55-100mL凍存袋24個(gè)/箱
OriGenCS750Freezing Bags,vol-max,80-190mL凍存袋24個(gè)/箱
OriGenCS1000N Freezing Bags,vol-max,120-275mL凍存袋,N型管路24個(gè)/箱
OriGenCS2000N Freezing Bags,vol-max,180-450mL凍存袋24個(gè)/箱
OriGenCS2000NP (廠家停產(chǎn))Freezing Bags,vol-max,180-450mL凍存袋12個(gè)/箱
OriGenCS30M95腔室Cryostore Multi-Chamber Bag 12個(gè)/箱
OriGenCS24M94腔室Cryostore Multi-Chamber Bag 12個(gè)/箱
    
培養(yǎng)袋貨號(hào)名稱規(guī)格
OriGenPL07-2GPermaLife Cell Culture Bags1個(gè)/包
OriGenPL30-2GPermaLife Cell Culture Bags1個(gè)/包
OriGenPL70-2GPermaLife Cell Culture Bags1個(gè)/包
OriGenPL120-2GPermaLife Cell Culture Bags1個(gè)/包
OriGenPL240-2GPermaLife Cell Culture Bags1個(gè)/包
OriGenPL240KPermaLife Cell Culture Bags,定制1個(gè)/包
OriGenPL325KPermaLife Cell Culture Bags,定制1個(gè)/包
OriGenPL325-2GPermaLife Cell Culture Bags1個(gè)/包
OriGenPL500P-2G PermaLife Cell Culture Bags(50個(gè)起訂)1個(gè)/包
OriGenPL750-2GPermaLife Cell Culture Bags1個(gè)/包
OriGenPL1000P-2G PermaLife Cell Culture Bags1個(gè)/包
OriGenPL2000P2-2G PermaLife Cell Culture Bags(50個(gè)起訂)1個(gè)/包
    
凍存液DMSO貨號(hào)名稱規(guī)格
OriGenCP-10Vial, 10ml CryoPur   : 100% DMSO12瓶/盒
OriGenCP-50Vial, 50ml CryoPur   : 100% DMSO6瓶/盒
OriGenCP-70Vial, 70ml CryoPur   : 100% DMSO6瓶/盒
OriGenCP-70-BagVial, 70ml CryoPur   : 100% DMSO70ml/瓶
OriGenCP-100Vial, 100ml CryoPur   : 100% DMSO6瓶/盒
OriGenSP-1010ml in 20ml syringe with stopcock and SCD tubing 1 unit 
OriGenSP-2020ml in 20ml syringe with stopcock and SCD tubing 1 unit 
OriGenSP-3030ml in 20ml syringe with stopcock and SCD tubing 1 unit 
OriGenSP-5050ml in 20ml syringe with stopcock and SCD tubing 1 unit 
    
混合液貨號(hào)名稱規(guī)格
OriGenCD-07CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection 
OriGenCD-35CryoPur-D™ : 55% DMSO, 5% Dextran-40w/v, remainder water for injection 
OriGenCD-50CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection6瓶/盒
OriGenCD-50-BagCryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection50ml/瓶
OriGenCD-100-BagCryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection100ml/瓶
OriGenSD-07CryoPur-D™ : 55% DMSO, 5% Dextran-40w/v, remainder water for injection,帶注射器 
OriGenSD-10CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection,帶注射器 
OriGenSDS-05A-B5ml 55% DMSO, 5% Dextran 40 w/v, remainder 0.9% saline + 2ml air in  20ml syringe with stopcock and SCD tubing  
OriGenSDS-15A15ml 55% DMSO, 5% Dextran 40 w/v, remainder 0.9% saline in 20ml syringe with stopcock and SCD tubing 
    
外袋貨號(hào)名稱規(guī)格
OriGenOW0419 10個(gè)/包
OriGenOW0909O-Wrap Overwrap Bags 外袋(適合CS25)10個(gè)/包
OriGenOW0918-SO-Wrap Overwrap Bags 外袋(適合CS25,CS50,PL07)10個(gè)/包
OriGenOW0918*O-Wrap Overwrap Bags 外袋(適合CS25,CS50,PL07)10個(gè)/包
OriGenOW0918-100SO-Wrap Overwrap Bags 外袋(適合CS25,CS50,PL07)100個(gè)/箱
OriGenOW1430O-Wrap Overwrap Bags 外袋(適合CS250,CS750,PL30,PL240)10個(gè)/包
OriGenOW1936O-Wrap Overwrap Bags 外袋(適合CS1000)10個(gè)/包
OriGenOW1950O-Wrap Overwrap Bags 外袋10個(gè)/包
OriGenOW2436O-Wrap Overwrap Bags 外袋(適合CS2000N)10個(gè)/包
*備注:OW0918*是由100個(gè)每箱的大包裝分裝成10個(gè)每包的小包裝 
    
熱合儀貨號(hào)名稱規(guī)格
OriGenCR6 Biosealer CR 6 熱合儀 1臺(tái)/箱
    
OriGenSZ1823SteriZip Overpouch(CS25, CS50 )10個(gè)/包
OriGenSZ2533SteriZip Overpouch(CS250, CS500, CS750 )10個(gè)/包
OriGenSZ3038SteriZip Overpouch(CS1000, CS2000)10個(gè)/包
    
OriGenSZ2533-2M SteriZip Overpouch,2.0毫米 
OriGenSZ2533P-1 SteriZip Overpouch,獨(dú)立包裝50個(gè)/包
OriGenSZ2533P-3SteriZip Overpouch,3個(gè)/小包45個(gè)/包
    
配件貨號(hào)名稱規(guī)格
OriGenS-F10One spike to one female luer with 10cm of tubing and pinch clamp, 100% DMSO Resistant50個(gè)/箱
OriGenRF-T156ml syringe on a female luer with 15cm sealed SCD tubing25個(gè)/箱
OriGen15-RF60-T60ml syringe on a female luer with 15cm sealed SCD tubing25個(gè)/箱
OriGen15-RF12-T12ml syringe on a female luer with 15cm sealed SCD tubing25個(gè)/箱
OriGen15-RF20-T20ml syringe on a female luer with 15cm sealed SCD tubing25個(gè)/箱
OriGenR3F-T153ml syringe on a female luer with 15cm sealed SCD tubing25個(gè)/箱
OriGen2S-P20Two spikes to one spike port with 20cm SCD tubing and pinch clamps20個(gè)/箱
OriGenS-38B12One spike to one 3/8″ barb with 12cm DMSO resistant tubing, 100% DMSO Resistant25個(gè)/箱
OriGenS-FFP12One spike to one spike port and two female luers with 12cm SCD tubing and pinch clamps20個(gè)/箱
OriGen4S-4M60Four spikes to four male luers with 60cm SCD tubing, roller and pinch clamps 
OriGenVSVVial spike with needle-free valve10個(gè)/包
OriGenS-M50One spike to male luer with 50cm SCD tubing and pinch clamp50個(gè)/箱
OriGenS-F50One spike to one female luer with 50cm SCD tubing and pinch clamp50個(gè)/箱
OriGenS-S50One spike to one spike with 50cm of SCD tubing12個(gè)/箱
OriGenCC2Cell Connect One spike and male luer to a 3-way stopcock, 60ml syringe and needle-free valve to four male luers with 70cm SCD tubing and pinch clamps12個(gè)/箱
OriGen12-F-MOne female luer to one male luer with 12cm tubing, 100% DMSO resistant50個(gè)/箱
OriGenCBSOne spike with needle-free valve, 100% DMSO Resistant 
    
淀粉貨號(hào)名稱規(guī)格
B.BraunL65116%Hetastarch in 0.9%Sodium Chloride羥乙基淀粉氯化鈉500ml/袋
B.BraunL65116%Hetastarch in 0.9%Sodium Chloride羥乙基淀粉氯化鈉12*500ml/箱
Hospira0409-7419-14-bag10% LMD in 0.9%Chlorde Injection右旋糖酐500ml/袋
    
    
袋子貨號(hào)名稱規(guī)格
OriGenEV120+F-M-12EVO Bag, 70-120ml Fill Volume, with luer connections and needle free valve with separate female to male tube set with 12cm of tubing20套/箱
OriGenEV1000NEVO Bag,100 -500ml Fill Volume, with one male luer with cap, two female luers  with caps, and a needle-free injection port12個(gè)/箱

OriGen

EV3000NEVO Bag, 500-2000ml Fill Volume, with one male luer with cap, two female luers with caps, and a needle-free injection port12個(gè)/箱

 

 


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