目錄:北京索萊寶科技有限公司>>ELISA試劑盒>>人 Human ELISA>> SEKH-0046Human IFN-gamma
| 供貨周期 | 現(xiàn)貨 | 規(guī)格 | 1600/48T; 2600/96T |
|---|---|---|---|
| 貨號 | SEKH-0046 | 主要用途 | 檢測人血清/血漿/細(xì)胞培養(yǎng)上清中干擾素的含量,定量檢測 |
Human IFN-gamma
| 中文名稱 | 人干擾素γ檢測試劑盒 |
|---|---|
| 英文名稱 | Human IFN-γ ELISA KIT |
| Reactivity | Human |
| Sample type | Serum, plasma, Cell culture supernatant |
| Sensitivity | 4 pg/ml |
| Detection Target | IFN-γ |
| Applications | Sandwich ELISA |
Human IFN-gamma
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IFN-γ is added to detect the captured IFN-γ protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm
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