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咖啡茵和代謝物分析
Caffeine and Metabolites
Conditions
Column: ACE 5 SuperC18
Dimensions: 150 x 4.6 mm
Part Number: EXL-1211-1546U
Mobile Phase:
A: 20 mM ammonium acetate pH 7.0 in H2O
B: 20 mM ammonium acetate pH 7.0 in MeCN/H2O (90:10 v/v)
Time(mins) | %B |
0 | 2 |
45 | 15 |
48 | 15 |
49 | 2 |
Post time 10 minutes
Flow Rate: 1 mL/min
Injection: 1 μL
Temperature: 60 °C
Detection: UV, 273 nm