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【ACE C8-300色譜柱】金魚(yú)魚(yú)腥草蛇毒色譜分析
Snake Venom from Crotalus Durissus Terrificus
Conditions
Column: ACE 3 C8-300
Dimensions: 50 x 4.6 mm
Part Number: ACE-212-0546
Mobile Phase:
A: 0.1% TFA in H2O
B: 0.1% TFA in MeCN/H2O (90:10 v/v)
Gradient:
Time(mins) | %B |
0 | 20 |
2 | 20 |
27 | 70 |
Flow Rate: 1.2 mL/min
Injection: 20 μL
Detection: UV, 214 nm
Sample: Lyophilised crude venom powder was solubilised (1 mg/mL) in 0.1% TFA. Resulting solutions were centrifuged
Analytes
1. Crotamine
2. Crotapotin
3. Phospholipase A2 (PLA2)