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【ACE Excel C18色譜柱】LC-MS/MS檢測(cè)類(lèi)固醇激素(內(nèi)源性)
Steroid Hormones (Endogenous) by LC-MS/MS
Conditions
Column: ACE Excel 2 C18
Dimensions: 50 x 2.1 mm
Part Number: EXL -101-0502U
Mobile Phase: A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeOH
Gradient:
Time(mins) | %B |
0.0 | 50 |
0.8 | 50 |
4.2 | 81 |
4.3 | 100 |
5.3 | 100 |
5.8 | 50 |
6.8 | 50 |
Flow Rate: 0.4 mL/min
Injection: 10 μL
Temperature: 40 °C
Detection: MS/MS
ESI in positive ion mode
Analytes
1. Aldosterone(m/z 361.4 315.4)
2. 21-Deoxycortisol(m/z 347.4 311.4)
3. 11-Deoxycortisol(m/z 347.4 97.0)
4. Androstenedione(m/z 287.3 97.0)
5. Testosterone(m/z 289.4 97.0)
6. DHEA(m/z 289.4 253.2)
7. 17α-Hydroxyprogesterone(m/z 331.4 97.0)
8. Progesterone(m/z 315.4 97.0)
9. Androsterone(m/z 291.3 255.4)