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【ACE UltraCore SuperC18色譜柱】真菌提取物中紫杉醇的LC-MS/MS分析
Taxol in Fungal Extract by LC-MS/MS
Conditions
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 150 x 2.1 mm
Part Number: CORE-25A-1502U
Mobile Phase: A: 0.5% formic acid in H2O
B: 0.5% formic acid in MeCN
Gradient:
Time(mins) | %B |
0.0 | 10 |
1.0 | 10 |
3.0 | 40 |
22.0 | 60 |
25.0 | 95 |
Flow Rate: 0.35 mL/min
Detection: Orbitrap Elite MS FT positive ion mode Collision induced dissociation isolation width 5 Da Normalised collision energy 32 eV Activation Q 0.25 Activation times 10 ms
Analyte
1. Taxol (Paclitaxel)