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【ACE Excel SuperC18色譜柱】三環(huán)類抗抑郁藥(梯度)色譜分析
Tricyclic Antidepressants (Gradient)
Conditions
Column: ACE Excel 2 SuperC18
Dimensions: 100 x 3.0 mm
Part Number: EXL -1011-1003U
Mobile Phase:
A: 20 mM ammonium formate pH 3.0 in H2O
B: 20 mM ammonium formate pH 3.0 in MeOH/H2O (9:1 v/v)
Gradient:
Time(mins) | %B |
0.0 | 50 |
6.0 | 70 |
7.0 | 70 |
7.5 | 50 |
Flow Rate: 1.2 mL/min
Injection: 2 μL
Temperature: 40 °C
Detection: UV, 260 nm
Analytes
1. Doxepin + isomer
2. Imipramine
3. Desipramine
4. Amitriptyline
5. Nortriptyline
6. Clomipramine