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【ACE C18色譜柱】酪氨酸、色氨酸,HPLC熒光檢測
Tyrosine, Tryptophan and Tramadol by HPLC with Fluorescence Detection
Conditions
Column:ACE 5 C18
Dimensions: 150 x 4.6 mm
Part Number: ACE-121-1546
Mobile Phase: A: 50 mM NaH2PO4 in H2O
B: MeCN/H2O (60:40 v/v)
Gradient:
Time(mins) | %B |
0.0 | 0 |
5.0 | 10 |
8.0 | 100 |
9.0 | 100 |
9.5 | 0 |
14.0 | 0 |
Flow Rate: 1 mL/min
Injection: 5 μL
Temperature: 25 °C
Detection: Fluorescence, λEx 280 nm, λEm 340 nm
Analytes
1. L -Tyrosine
2. L -Tryptophan
3. Tramadol