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【ACE Excel C18-PFP色譜柱】血清中25-羥基維生素D的LC-MS/MS分析
25-Hydroxy Vitamin D in Serum by LC-MS/MS
Conditions
Column: ACE Excel 2 C18-PFP
Dimensions: 100 x 2.1 mm
Part Number: EXL -1010-1002U
Mobile Phase: A: 2 mM ammonium acetate,
0.1% formic acid in H2O
B: 0.1% formic acid in MeOH
Gradient:
Time(mins) | %B |
0.0 | 75 |
3.0 | 100 |
4.0 | 100 |
Flow Rate: 0.4 mL/min
Injection: 15 μL
Temperature: 40 °C
Detection: Quattro Premier XE triple quad MS MRM positive ESI mode
Desolvation temperature: 450 °C
Analytes
1a. 25-OH Vitamin D2
(m/z 395.5 269.5)
1b. 25-OH Vitamin D2
(m/z 395.5 119.2)
2. d6-25-OH Vitamin D3 (IS)
(m/z 389.6 263.5)
3a. 25-OH Vitamin D3
(m/z 383.5 257.5)
3b. 25-OH Vitamin D3
(m/z 383.5 107.2)