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【ACE UltraCore SuperC18色譜柱】LC-MS法提取微生物提取物
Microbial Extract by LC-MS
Dionex 3000RS UHPLC system coupled with Bruker MaXis Q-TOF MS
UltraCore SuperC18 2.5um, 150 x 2.1mm
Gradient analysis
A = 0.1% formic acid in water
B = 0.1% formic acid in acetonitrile
Time (mins) %B Time (mins) %B
0 5 20 100
5 5 25 100
Flow rate: 0.2ml/min
Electrospray MS (positive mode)
Source: End plate offset: -500V,
Capillary: -4500V
Nebuliser gas (N2) at 1.6 bar
Drying gas (N2) temp.: 180°C
Collision energy: 5.0eV
Collision RF: 600Vpp