S1000-biocolor 膠原檢測(cè)試劑盒（Sircol Soluble Collagen Assay ）

Biocolor 可溶性膠原檢測(cè)試劑盒
Biocolor Sircol Soluble Collagen Assay 

    采用與膠原特異性結(jié)合的Sircol染料，主要用于定量檢測(cè)哺乳動(dòng)物組織中抽提的酸溶性膠原和體外培養(yǎng)哺乳動(dòng)物細(xì)胞釋放到培養(yǎng)基中的膠原。

    溶于酸、胃蛋白等的可溶性膠原均可檢測(cè)，具體可檢測(cè)的膠原類型為I-V型，VI-XIV型膠原也可檢測(cè)，但由于難以制備標(biāo)準(zhǔn)曲線，還無法定量。Sircol染料不能區(qū)分不同類型的膠原，但可與膠原的[Gly-X-Y]n螺旋結(jié)構(gòu)特異性地結(jié)合。隨著膠原變性為凝膠，該染料與膠原的結(jié)合力減弱。非脊椎動(dòng)物與膠原由于變性溫度較低、羥脯氨酸殘基較少等原因，結(jié)合染料的能力較弱，需要制備種屬特異性的純化標(biāo)準(zhǔn)品，以繪制標(biāo)準(zhǔn)曲線。
  
實(shí)驗(yàn)步驟

1. 繪制標(biāo)準(zhǔn)曲線
2. 待測(cè)樣本的制備：取50ul樣本容易調(diào)整體積到100ul進(jìn)行反應(yīng)。根據(jù)反應(yīng)的結(jié)果對(duì)樣本濃度進(jìn)行調(diào)整，使其在標(biāo)準(zhǔn)曲線范圍內(nèi)；
3. 膠原—染料復(fù)合物的獲得：加入1ml Sircol染料，混勻離心后，既得膠原—染料復(fù)合物，棄上清，去掉未結(jié)合的染料
4. 加入1ml 染料釋放劑，將膠原—染料復(fù)合物中的染料釋放出來
5. 吸光度測(cè)定：用分光光度計(jì)540nm出定量測(cè)定?；蚴褂胏olorimeter測(cè)定

產(chǎn)品訂購 
    Product Code: S1000    120 assays 
    Product Code: S5000    475 assays

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