大鼠N-乙酰-β-D-氨基葡萄糖苷酶ELISA Kit

大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit

產(chǎn)品類型:
ELISA Kit

產(chǎn)品名稱:

大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit

英文名稱:

Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

貨號:
大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit別名:
ENC-1AS, N-acetyl-beta-glucosaminidase|hexosaminidase B, Beta-hexosaminidase subunit beta(HEXB)
規(guī)格:
96T
中文價格:
大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit種屬:
Rat
其他種屬:
Human Mouse Pig
待測物名稱:
N-acetyl-β-D-glucosaminidase,NAG
縮寫:
NAG
檢測范圍:
1.56 mIU/ml-100 mIU/ml
靈敏度:
0.39 mIU/ml
反應(yīng)時間:
1-5h
所需樣本體積:
50-100ul
檢測波長:
450 nm

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated 大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kitantibody specific for NAG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAG bound in the initial step. The color development is stopped and the intensity of the color is measured.