ADP核糖基化因子結(jié)合蛋白抗體 返回列表頁

英文名稱 ARFBP1
中文名稱 ADP核糖基化因子結(jié)合蛋白抗體
別    名 ARF binding protein 1; ARF BP1; ARF-binding protein 1; ARF-BP1; BJ-HCC-24 tumor antigen; E3 ubiquitin protein ligase HUWE1; E3 ubiquitin-protein ligase HUWE1; HECT; HECT domain protein LASU1; HECT UBA and WWE domain containing protein 1; HectH9; Homologous to E6AP carboxyl terminus homologous protein 9; HUWE; Huwe1; HUWE1_HUMAN; Ib772; KIAA0312; KIAA1578; Large structure of UREB1; LASU1; Mcl 1 ubiquitin ligase E3; Mcl-1 ubiquitin ligase E3; MULE; UBA and WWE domain-containing protein 1; Upstream regulatory element-binding protein 1; URE B1; URE-B1; URE-binding protein 1; UREB 1; UREB1.
ADP核糖基化因子結(jié)合蛋白抗體    
說 明 書 0.2ml  
研究領(lǐng)域 細(xì)胞生物  信號轉(zhuǎn)導(dǎo)  轉(zhuǎn)運(yùn)蛋白  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, 
產(chǎn)品應(yīng)用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 482kDa
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human ARF6
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation.

Function:
GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension.

Subunit:
Interacts with isoform p14ARF of CDKN2A which strongly inhibits HUWE1 ubiquitin ligase activity. Interacts with MYCN, POLB and CDC6.

Subcellular Location:
Cytoplasm. Nucleus. Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells.

Tissue Specificity:
Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas.

Post-translational modifications:
Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation.
Phosphorylated upon DNA damage, probably by ATM or ATR.

DISEASE:
Defects in HUWE1 are the cause of mental retardation syndromic X-linked Turner type (MRXST) [MIM:300706]; also known as mental retardation and macrocephaly syndrome. MRXST shows clinical variability. Associated phenotypes include macrocephaly and variable contractures.
A chromosomal microduplication involving HUWE1 and HSD17B10 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general inlectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, inlectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.

Similarity:
Belongs to the TOM1/PTR1 family.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 UBA domain.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 WWE domain.

Gene ID:
10075

Database links:

Entrez Gene: 465650 Chimpanzee

Entrez Gene: 10075 Human

Entrez Gene: 59026 Mouse

SwissProt: Q7Z6Z7 Human

SwissProt: Q5BMM7 Mouse

SwissProt: Q7TMY8 Mouse

Unigene: 136905 Human

Unigene: 27372 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.xy-2413R PAX7配對盒基因7抗體
xy-0655R PAK5激活激酶PAK7抗體
xy-0420R PIBF孕激素誘導(dǎo)阻斷因子抗體
xy-0754R PCNA增殖細(xì)胞核抗原抗體
xy-0491R PCNA增殖細(xì)胞核抗原抗體
xy-0665R PIWIL1piwi樣1蛋白抗體
xy-0196R PDGF-A血小板源性生長因子A抗體
xy-0508R PRL泌乳素抗體
xy-0185R PDGF-B血小板源性生長因子-B抗體
xy-0231R PDGFRA血小板源性生長因子受體A抗體(PDGFRα)
xy-0232R PDGF Receptor beta血小板源性生長因子受體B抗體(PDGFRβ)
xy-0682R PDK4丙酮酸脫氫酶激酶4抗體
xy-0731R PEDF色素上皮源性因子抗體
xy-1084R PEG10肝癌高表達(dá)基因抗體
xy-0689R PEPT1腸道肽轉(zhuǎn)運(yùn)蛋白1/小肽轉(zhuǎn)運(yùn)蛋白1抗體
xy-0584R SLC15A2腸道肽轉(zhuǎn)運(yùn)蛋白2抗體
xy-11146R PCDHGA3原鈣粘蛋白γA3抗體
xy-1316R PDGFBB血小板源性生長因子BB抗體
xy-0923R PDX1胰島素促進(jìn)因子抗體（胰十二指腸同源異型盒蛋白）
xy-0905R Acetyl-p53(K382)乙?；疨53(Lys382)抗體
xy-3737R Phospho-PPAR Gamma (ser112)磷酸化過氧化酶活化增生受體γ抗體
xy-3705R phospho-P53(Ser33)磷酸化腫瘤抑制基因P53抗體
xy-0198R PACAP receptor腺苷酸環(huán)化酶激活肽受體1抗體
xy-0318R PADI4關(guān)節(jié)炎相關(guān)基因抗體
xy-1119R PAF血小板活化因子抗體
xy-0828R Thrombin receptor蛋白酶激活受體-1抗體