CD44 （CD44） 抗體 （Biotin） 返回列表頁(yè)

CD44 (CD44) 抗體 (Biotin)

抗原
CD44 (CD44)
別名 AU023126, AW121933, AW146109, HERMES, Ly-24, Pgp-1, CD44A, METAA, RHAMM, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1
適用
其他選擇 Murine
Human(634),Murine (315),Rat (Rattus) (137),Dog (Canine) (76),Pig (Porcine) (64),Bovine (55),Chicken (33),Monkey (16),Cat (12),Horse (Equine) (12),Sheep (Ovine) (9),Rabbit (8),Baboon (7),Goat (3),Rat (Meriones) (1)
宿主
其他選擇 Rat
Mouse(518),Rat (200),Rabbit (171),Goat (9),Chicken (2),Human (1),Sheep (1)
克隆類型 (克隆位點(diǎn))
Monoclonal (KM81)
標(biāo)記
其他選擇 Biotin
FITC(144),Biotin (81),PE (69),APC (29),HRP (9),Alexa Fluor 488 (7),Alexa Fluor 555 (7),Alexa Fluor 647 (7),Cy3 (7),Cy5 (7),Cy5.5 (7),Cy7 (7),SPRD (7),PE-Cy7 (5),PerCP (4),Alexa Fluor 700 (3),Cy5,PE (3),PE,Cy5 (3),APC eFluor 780 (2),Alkaline Phosphatase (AP) (2),Allophycocyanin (2),DyLight 488 (2),DyLight 550 (2),DyLight 594 (2),DyLight 650 (2),DyLight 680 (2),DyLight 755 (2),EFluor 450 (2),EFluor 605NC (2),PE,Cy7 (2),PE-Cy5 (2),PerCP,Cy5.5 (2),Magnetic Particles (1),PE-Cy3 (1),PE-Cy5.5 (1),PE-Dyomics 647 (1),Sepharose (1)
應(yīng)用范圍
其他選擇 Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
Flow Cytometry (FACS)(564),Western Blotting (WB) (283),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (216),Immunoprecipitation (IP) (155),Immunohistochemistry (Frozen Sections) (IHC (fro)) (149),ELISA (127),Immunohistochemistry (IHC) (121),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (67),Immunofluorescence (IF) (54),Immunocytochemistry (ICC) (46),Functional Studies (Func) (13),Cytotoxicity Test (CyTox) (6),Blocking Reagent (BR) (5),Intracellular Staining (ICS) (5),Radioimmunoassay (RIA) (3),Immunoassay (IA) (2),Neutralization (Neut) (2),Enzyme Immunoassay (EIA) (1),Fluorescence Microscopy (FM) (1),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1),Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
[Pubmed] 4 references available
規(guī)格 0.1 mg
發(fā)貨至 中國(guó) (Change)
CD44 (CD44) 抗體 (Biotin)

目標(biāo)詳細(xì)情況

顯示 隱藏
別名 CD44
背景 CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Alternate names: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
基因ID 12505
NCBI Accession NP_001034240
UniProt P15379
研究領(lǐng)域 Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle

使用細(xì)節(jié)

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使用細(xì)節(jié)

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CD44 (CD44) 抗體 (Biotin)應(yīng)用備注 Flow Cytometry. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
實(shí)驗(yàn)流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue DistributionMouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Rat IgG2aResults - Strain Distribution: Cell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsStrains Tested: BALB/c, CBA/J, C3H/He, C57BL/6, SWRPositive: BALB/c, CBA/J, C3H/He, C57BL/6, SWR
限制 For Research Use only

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貯存及處理

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狀態(tài) Liquid
濃度 0.1 mg/mL
緩沖液 PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
儲(chǔ)存液 Sodium azide
注意事項(xiàng) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CD44 (CD44) 抗體 (Biotin)注意事項(xiàng) Avoid repeated freezing and thawing.
儲(chǔ)存條件 4 °C/-20 °C
儲(chǔ)存方法 Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
有效期 12 months

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