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CD19 Molecule (CD19) 抗體

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CD19 Molecule (CD19) 抗體的概念:
Z初有人用電泳證明血清中抗體活性在γ球蛋白部分,故曾把抗體統(tǒng)稱為丙種(γ)球蛋白。后來發(fā)現(xiàn),抗體并不都在γ區(qū);并且位于γ區(qū)的球蛋白,也不一定都具有抗體活性,規(guī)格:0.1ml/100µg、0.2ml/200µg

CD19 Molecule (CD19) 抗體

抗原
CD19 Molecule (CD19)
別名 CD19, AW495831, B4, CVID3
適用
其他選擇 Human
Human(431),Murine (285),Rat ((Rattus)) (41),Guinea Pig (36),Pig (Porcine) (36),Bovine (24),Dog (Canine) (24),Monkey (2)
宿主
其他選擇 Mouse
Mouse(369),Rat (149),Rabbit (144),Goat (3),Chicken (2)
克隆類型 (克隆位點)
單克隆 (LT19)
標記
其他選擇 PerCP
PE(78),FITC (77),Biotin (59),APC (38),Cy5 (11),Cy5,PE (10),PerCP (10),PE,Cy5 (9),SPRD (9),PE-Cy5 (8),Alexa Fluor 647 (7),HRP (7),PE-Cy7 (7),PE-Cy5.5 (6),Alexa Fluor 350 (5),Alexa Fluor 488 (5),Alexa Fluor 555 (5),Cy3 (5),Cy5.5 (5),Cy7 (5),PerCP,Cy5.5 (5),APC-Cy7 (4),Cy7,PE (4),APC-Cy5.5 (2),Alexa Fluor 700 (2),Allophycocyanin (2),DyLight 488 (2),DyLight 550 (2),DyLight 594 (2),DyLight 650 (2),DyLight 680 (2),DyLight 755 (2),EFluor 450 (2),PE,Cy7 (2),APC,Cy5.5 (1),APC,Cy7 (1),Alkaline Phosphatase (AP) (1),CF405M (1),FITC,PE (1),PE,Cy5.5 (1),PE,DY647 (1),PE-DyLight 594 (1),PE-Dyomics 590 (1),PE-Dyomics 647 (1),Texas Red (TR) (1)
應用范圍
其他選擇 Flow Cytometry (FACS)
Flow Cytometry (FACS)(477),Western Blotting (WB) (106),Immunoprecipitation (IP) (102),Immunohistochemistry (IHC) (82),ELISA (62),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (52),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (50),Immunohistochemistry (Frozen Sections) (IHC (fro)) (41),Immunofluorescence (IF) (31),Immunocytochemistry (ICC) (26),Functional Studies (Func) (15),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (10),Enzyme Immunoassay (EIA) (4),Fluorescence Microscopy (FM) (1),Immunoassay (IA) (1),Proximity Ligation Assay (PLA) (1)
[Pubmed] 8 references available
規(guī)格 100 tests
發(fā)貨至 中國 (更改)
發(fā)貨時間 23至29個工作日

CD19 Molecule (CD19) 抗體

產(chǎn)品細節(jié)

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免疫原 Daudi human Burkitt lymphoma cell line
克隆位點 LT19
亞型 IgG1
特異性 The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it is lost on plasma cells.
產(chǎn)品特性 The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

目標詳細情況

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CD19 Molecule (CD19) 抗體目標詳細情況

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別名 CD19
背景 CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
研究領域 Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

使用細節(jié)

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使用細節(jié)

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應用備注 The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
限制 For Research Use only

貯存及處理

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貯存及處理

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溶解方式 No reconstitution is necessary.
緩沖液 The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
儲存液 Sodium azide
注意事項 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediay. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
CD19 Molecule (CD19) 抗體注意事項 Do not freeze.
Avoid prolonged exposure to light.
儲存條件 4 °C
儲存方法 Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

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