Neutrophil Cytosolic Factor 4, 40kDa （NCF4） （Inter 返回列表頁

Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) 抗體

抗原
Neutrophil Cytosolic Factor 4, 40kDa (NCF4)
別名 p40phox, AI451400, NCF, P40PHOX, SH3PXD4
抗原表位
其他選擇 Internal Region
pThr154(17),C-Term (10),Internal Region (7),N-Term (3),AA 1-100 (2),AA 1-339 (2),AA 260-289 (2),Middle Region (2),AA 1-101 (1),AA 1-298 (1),AA 120-169 (1),Arg149 (1),Thr117 (1)
適用
其他選擇 Human
Human(77),Murine (43),Rat ((Rattus)) (33),Dog (Canine) (30),Guinea Pig (24),Bovine (14),Pig (Porcine) (12),Rabbit (2)
宿主
其他選擇 Goat
Rabbit(66),Mouse (8),Goat (6)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Alexa Fluor 350(2),Alexa Fluor 488 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Biotin (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),FITC (2),HRP (2)
實驗類型 Peptide ELISA
應(yīng)用范圍
其他選擇 ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB)(46),ELISA (23),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Immunohistochemistry (IHC) (20),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17),Flow Cytometry (FACS) (5),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (3),Enzyme Immunoassay (EIA) (2),Immunoassay (IA) (2),Immunocytochemistry (ICC) (1),Immunofluorescence (IF) (1),Immunoprecipitation (IP) (1)
[Pubmed] 1 reference available
規(guī)格 100 μg
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Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) 抗體

產(chǎn)品細(xì)節(jié)

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免疫原 Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2, NP_038202.1.
特異性 This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
交叉反應(yīng) Dog (Canine)
純化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) 抗體目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 NCF4 / P40PHOX
背景 Synonyms: NCF4, P40PHOX, neutrophil cytosolic factor 4, 40kDa, CTA-833B7.1, MGC3810, NCF, SH3PXD4, OTTHUMP00000028736, OTTHUMP00000043756, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD)
基因ID 4689
NCBI Accession NP_038202, NP_000622
研究領(lǐng)域 Autophagy

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 38 kDa band observed in lysates of cell line Daudi (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
IHC: In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration: 2-4 µg/mL.
限制 For Research Use only

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狀態(tài) Liquid
緩沖液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
儲存液 Sodium azide
Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) 抗體注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項 Minimize freezing and thawing.
儲存條件 -20 °C
儲存方法 Aliquot and store at -20 °C.

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