Low Density Lipoprotein Receptor-Related Protein 6 返回列表頁

Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) (Internal Region) 抗體

抗原
Low Density Lipoprotein Receptor-Related Protein 6 (LRP6)
別名 lrp6-A, Xlrp6, adcad2, lrp6, LRP6, ADCAD2, C030016K15Rik, Cd, Gw
抗原表位
其他選擇 Internal Region
pSer1490(14),pThr1479 (13),C-Term (12),Internal Region (4),AA 1546-1560 (3),AA 1531-1560 (2),AA 1171-1469 (1),AA 1221-1439 (1),AA 1525-1575 (1),AA 20-1368 (1),AA 755-1060 (1),AA 981-1213 (1)
適用
其他選擇 Human
Human(70),Murine (38),Dog (Canine) (37),Bovine (36),Pig (Porcine) (36),Rat ((Rattus)) (26),Chicken (12),Guinea Pig (12),Purple Sea Urchin (Strongylocentrotus purpuratus) (1)
宿主
其他選擇 Goat
Rabbit(67),Goat (4),Mouse (1),Sheep (1)
克隆類型
多克隆
標記
其他選擇 非結(jié)合性
Biotin(4),FITC (4),HRP (4),Alexa Fluor 350 (3),Alexa Fluor 488 (3),Alexa Fluor 555 (3),Alexa Fluor 647 (3),Cy3 (3),Cy5 (3),Cy5.5 (3),Cy7 (3),APC (1),Alkaline Phosphatase (AP) (1),PE (1)
實驗類型 Peptide ELISA
應(yīng)用范圍
其他選擇 ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB)(38),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (30),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21),ELISA (16),Immunohistochemistry (IHC) (13),Immunofluorescence (Cultured Cells) (IF (cc)) (10),Enzyme Immunoassay (EIA) (3),Immunocytochemistry (ICC) (3),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (3),Immunoprecipitation (IP) (3),Immunofluorescence (IF) (2),Immunohistochemistry (Frozen Sections) (IHC (fro)) (2),Dot Blot (DB) (1),Flow Cytometry (FACS) (1)
[Pubmed] 2 references available
規(guī)格 100 μg
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發(fā)貨時間 24至29個工作日

(LRP6) (Internal Region) 抗體

產(chǎn)品細節(jié)

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免疫原 Peptide with sequence CMTSVATAKGYTSDL, from the internal region of the protein sequence according to NP_002327.2.
特異性 This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.
交叉反應(yīng) Murine, Rat ((Rattus)), Dog (Canine)
純化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

目標詳細情況

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(LRP6) (Internal Region) 抗體目標詳細情況

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別名 LRP6
背景 Synonyms: LRP6, low density lipoprotein receptor-related protein 6, low density lipoprotein-related protein 6
基因ID 4040, 16974
NCBI Accession NP_002327
研究領(lǐng)域 Stem Cells, Wnt

使用細節(jié)

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使用細節(jié)

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應(yīng)用備注 ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 50 kDa and 35 kDa in Human Adipose lysates after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180 kDa according to NP_002327.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration: 4-6 µg/mL.
限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Liquid
緩沖液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
儲存液 Sodium azide
(LRP6) (Internal Region) 抗體注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事項 Minimize freezing and thawing.
儲存條件 -20 °C
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