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MECP2 抗體

抗原
Methyl CpG Binding Protein 2 (Rett Syndrome) (MECP2)
別名 wu:fk96a04, zgc:111857, 1500041B07Rik, BB130002, D630021H01Rik, Mbd5, WBP10, AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT
抗原表位
其他選擇 AA 469-486
N-Term(18),pSer80 (15),Ser421 (13),pSer421 (9),C-Term (7),pSer292 (7),AA 3-33 (6),AA 406-435 (6),Ser80 (6),AA 11-25 (4),AA 81-170 (4),AA 1-486 (3),AA 310-360 (3),AA 181-195 (2),AA 1-15 (1),AA 1-232 (1),AA 2-211 (1),AA 469-486 (1),AA 66-282 (1),Internal Region (1),Middle Region (1)
適用
其他選擇 Human, Murine, Rat ((Rattus))
Human(114),Murine (36),Rat ((Rattus)) (30),Dog (Canine) (15),Chicken (12),Bovine (12),Pig (Porcine) (12),Mammalian (2),Rabbit (2),Xenopus laevis (1)
宿主
其他選擇 Rabbit
Rabbit(107),Mouse (11),Chicken (2)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(8),FITC (8),HRP (8),APC (7),Alkaline Phosphatase (AP) (7),PE (7),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Western Blotting (WB)
Western Blotting (WB)(84),ELISA (56),Immunohistochemistry (IHC) (25),Flow Cytometry (FACS) (20),Dot Blot (DB) (17),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Enzyme Immunoassay (EIA) (4),Immunoassay (IA) (3),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (3),Immunocytochemistry (ICC) (2),Immunofluorescence (IF) (2),Immunoprecipitation (IP) (2),Chromatin Immunoprecipitation (ChIP) (1),Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
[Pubmed] 4 references available
規(guī)格 0.1 mg
發(fā)貨至 中國(guó) (更改)
發(fā)貨時(shí)間 29至35個(gè)工作日

MECP2 抗體

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Synthetic peptides corresponding to aa 469-486 of MeCP2 protien
特異性 This antibody detects a single band of approximay 45?kDa of MeCP2. It does not cross react with other histones or other DNA binding protiens.
交叉反應(yīng) （詳細(xì)） Species reactivity (tested):Human, mouse, rat
純化方法 Affinity chromatography

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 MeCP2
背景 The epigenetic information encoded as methylated CpG dinucleotides to the transcriptional machinery is transferred by a multifunction methylated DNA binding protein called Methylated CpG biding protein 2. Mutation in MeCP2 protein led to debilitating neurogenerative X-linked autism-spectrum neurodevelopmental disease Rhett syndrome (RTT). Several functional domains have been identified on the MeCP2 protein including Methylated non-methylated DNA binding domains in the first 294 amino acids and a chromatin binding motif near the C-terminal DNA region of the protein (1). The genetic and epigenetic defects led to reduced expression of MeCP2 protein in brain of was autism patients, it may be an important factor in the complex etiology of this disease (2). The gene is located on Xq28 chromosome. Defects in the MeCP2 gene care caused by Angelman syndrome also known as happy puppet syndrome which a neuron-developmental disorder characterized by severe mental retardation, absent of speech, ataxia, sociable affects and dysmorphic facial features similar to Rhett syndrome clinical feature. Rett syndrome (RS) is an X-linked neuron-developmental disorder and the second most common cause of genetic mental retardation in females. Different mutations in MECP2 are found in up to 95?% of typical cases of RS. The onset of this disease is caused by non progressive encephalopathy with neonatal onset. The defects in MeCP2 is cause of X-linked mental retardation, Autism 3 (AUTSX3) about 80?% of the Rhett syndrome cases have mutational defects in the MeCP2 gene. Expression of this protein sprout generation of neuron and reversal of neurological defects in a moue model of Rett syndrome (3). MeCP2 protein has 2 AT hook DNA biding domains, a transcriptional repression domain (TRD) and one MBD or Methyl CpG binding domain on the amino terminal end (1-96). Methyl CGp binding protein 2 gene is located on fragile X chromosome and this chromosomal protein binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. The binding is not influenced by sequences flanking the methyl-CpGs. Upon binding it mediates transcriptional repression through interaction with histone deacetylase and co repressor SIN3A. MeCP2, is responsible for transcriptional repression of the leukosialin gene by repression of the Sp1-activated trasnacription. Methyl-CpG-binding protein MeCP2 represses Sp1-activated transcription of the human leukosialin gene when the promoter is methylated (3).Synonyms: MeCP-2 protein, Methyl-CpG-binding protein 2
基因ID 4204
NCBI Accession NP_004983
UniProt P51608
研究領(lǐng)域 Neurology

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA: < 1: 10,000. Western blot: > 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

貯存及處理

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貯存及處理

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濃度 0.5 - 0.95 mg/mL
緩沖液 Stabilization buffer
注意事項(xiàng) Avoid repeated freezing and thawing.
儲(chǔ)存條件 4 °C/-20 °C
儲(chǔ)存方法 Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

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