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當(dāng)前位置:上海信裕生物科技有限公司>>抗體>>一抗>> Interleukin 4 (IL4) 抗體
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更新時(shí)間:2018-03-15 13:17:56瀏覽次數(shù):422次
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抗原
Interleukin 4 (IL4)
別名 IL4, IL-4, BCGF-1, BCGF1, BSF-1, BSF1, Il-4, Il4e12, IL2
適用
其他選擇 Murine
Human(220),Murine (148),Rat ((Rattus)) (32),Bovine (11),Pig (Porcine) (7),Dog (Canine) (6),Rabbit (4),Goat (2),Horse (Equine) (2),Musid (2),Sheep (Ovine) (2),Rhesus Monkey (1),Wild boar (Sus scrofa) (1)
宿主
其他選擇 Rabbit
Rabbit(147),Rat (139),Mouse (111),Sheep (6),Goat (3),Guinea Pig (3)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(52),PE (29),FITC (16),APC (12),HRP (8),Alexa Fluor 488 (4),Allophycocyanin (3),PerCP (3),Alexa Fluor 350 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),Cy7,PE (2),DyLight 488 (2),DyLight 550 (2),DyLight 594 (2),DyLight 650 (2),DyLight 755 (2),PE,Cy7 (2),Alkaline Phosphatase (AP) (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
ELISA(204),Western Blotting (WB) (200),Flow Cytometry (FACS) (126),Immunohistochemistry (IHC) (50),Neutralization (Neut) (35),Immunohistochemistry (Frozen Sections) (IHC (fro)) (29),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27),Immunoprecipitation (IP) (26),Enzyme Immunoassay (EIA) (23),Immunocytochemistry (ICC) (23),ELISpot (21),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Functional Studies (Func) (19),Immunofluorescence (IF) (18),Blocking Antibody (Inhibition) (8),Intracellular Staining (ICS) (8),ELISA (Detection) (4),Immunoassay (IA) (4),Transfected cell culture (TCC) (4),Dot Blot (DB) (3),ELISA (Capture) (3),Blocking Reagent (BR) (1),ELISpot (Capture) (1),ELISpot (Detection) (1),Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (1),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1),Radioimmunoassay (RIA) (1)
[Pubmed] 2 references available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時(shí)間 29至35個(gè)工作日
Interleukin 4 (IL4) 抗體
產(chǎn)品細(xì)節(jié)
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產(chǎn)品細(xì)節(jié)
顯示 隱藏
免疫原 Highly pure (>98%) recombinant mIL-4 (murine Interleukin-4).
特異性 Recognises Mouse Interleukin-4 (IL-4).
純化方法 Affinity chromatography.
目標(biāo)詳細(xì)情況
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Interleukin 4 (IL4) 抗體目標(biāo)詳細(xì)情況
顯示 隱藏
別名 Interleukin-4 / IL4
背景 IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
基因ID 16189
NCBI Accession NP_067258
UniProt P07750
Interleukin 4 (IL4) 抗體使用細(xì)節(jié)
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使用細(xì)節(jié)
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應(yīng)用備注 Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-4 (7.5?ng/mL), aconcentration of 0.25-0.40?μg/mL of this antibody is required. ELISA: (Indirect): To detect mIL-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0?μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4?ng/well of recombinant mIL-4. (Sandwich): To detect mIL-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0?μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IL-4 (PP011B) as a detectionantibody, allows the detection of at least 0.2-0.4?ng/well of recombinant mIL-4. Western Blot: To detect mIL-4 by Western Blot analysis this antibody can be used at a concentration of
限制 For Research Use only
貯存及處理
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貯存及處理
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溶解方式 Restore in sterile water to a concentration of 0.1-1.0?mg/mL.
濃度 1.0 mg/mL
緩沖液 PBS,?pH 7.2 without preservatives.
儲(chǔ)存液 Without preservative
注意事項(xiàng) Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
儲(chǔ)存條件 4 °C/-20 °C
儲(chǔ)存方法 Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
xy-2703R CD170唾液酸結(jié)合性免疫球蛋白樣凝集素5抗體
xy-2705R CD329CD329抗體
xy-2479R CCL14嗜酸粒細(xì)胞趨化蛋白14抗體
xy-9801R C1orf1571號(hào)染色體開放閱讀框57抗體
xy-9805R C2orf182號(hào)染色體開放閱讀框18抗體
xy-12925R COX6c細(xì)胞色素c氧化酶6抗體
xy-2483R CCL24嗜酸粒細(xì)胞趨化蛋白24抗體
xy-2480R CCL15巨噬細(xì)胞炎性蛋白15
xy-2482R CCL23/MIP3巨噬細(xì)胞炎性蛋白抗體
xy-2517R CD72/Ly-32CD72蛋白抗體
xy-9306R CEP128中心體蛋白128抗體
xy-9305R CENPO著絲粒蛋白O抗體
xy-9824R C3orf173號(hào)染色體開放閱讀框17抗體
xy-6310R Caveolin-2細(xì)胞質(zhì)膜微囊蛋白-2抗體
xy-7523R phospho-Caveolin-2(Tyr19)磷酸化細(xì)胞質(zhì)膜微囊蛋白-2抗體
xy-9822R C3orf243號(hào)染色體開放閱讀框24抗體
xy-4871R Complement C3 beta chain補(bǔ)體C3b抗體
xy-4873R Complement C3b alpha' chain補(bǔ)體C3b-α鏈抗體
xy-4816R CD3ECD3抗體
xy-15555R CENPC1著絲粒蛋白C1抗體
xy-9128R CDCA7細(xì)胞分裂周期相關(guān)蛋白
xy-4745R CSFV豬瘟病毒抗體
xy-7341R C16orf7216號(hào)染色體開放閱讀框72抗體
xy-10454R Complement C4 gamma chain補(bǔ)體C4 γ 鏈蛋白抗體
xy-10453R C4d-A補(bǔ)體C4d-A蛋白抗體
xy-10452R C4b-A補(bǔ)體C4b-A蛋白抗體
xy-10462R Phospho-CD18 (Thr758)磷酸化整合素β2/Integrin β2抗體
xy-10461R Phospho-CD18 (Ser756)磷酸化整合素β2/Integrin β2抗體
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