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DBN1抗體

抗原
Drebrin 1 (DBN1)
別名 LOC733967, D0S117E, DBN1
抗原表位
其他選擇 AA 324-343
AA 632-649(7),AA 1-326 (6),N-Term (6),AA 254-272 (4),C-Term (4),AA 324-343 (3),AA 1-649 (1),AA 209-300 (1),AA 22-42 (1),AA 31-262 (1),AA 401-596 (1),AA 482-649 (1),Internal Region (1)
適用
其他選擇 Bovine, Human, Murine, Rat ((Rattus))
Human(44),Rat ((Rattus)) (19),Murine (18),Bovine (16),Chicken (15),Dog (Canine) (15),Pig (Porcine) (11)
宿主
其他選擇 Guinea Pig
Rabbit(22),Mouse (20),Guinea Pig (7),Sheep (1)
克隆類型
多克隆
標記
其他選擇 非結(jié)合性
PE(3),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Biotin (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),FITC (1),HRP (1)
應用范圍
其他選擇 Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB)(35),Immunoprecipitation (IP) (15),Immunofluorescence (IF) (14),Immunohistochemistry (Frozen Sections) (IHC (fro)) (11),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11),Immunohistochemistry (IHC) (10),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (9),ELISA (6),Flow Cytometry (FACS) (6),Immunocytochemistry (ICC) (5),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1)
[Pubmed] 3 references available
規(guī)格 0.1 mL
發(fā)貨至 中國 (更改)
發(fā)貨時間 29至35個工作日

DBN1抗體

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

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免疫原 Synthetic Human peptide (aa 324-343) coupled to KLH.
特異性 The antiserum specifically reacts with Drebrin isoforms E2 and A, widespread actin-associating proteins of 70 kDa (SDS-PAGE mobility shows Mr 120,000). Reacts with PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80, B1, B16 (mouse melanoma cells).
純化方法 Serum

目標詳細情況

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目標詳細情況

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別名 Drebrin
背景 Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative, also positive: regenerating epithelium during wound healing, basal cell carcinoma, skin melanoma), specific types of endothelia and smooth muscle, especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis. Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle.Synonyms: D0S117E, DBN-1, DBN1, DKFZp434D064, Developmentally-regulated brain protein, drebrin 1
基因ID 1627
NCBI Accession NP_004386
UniProt Q16643
研究領域 Cancer, Cytoskeleton, Microfilaments, Alzheimer's Disease

使用細節(jié)

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使用細節(jié)

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應用備注 Immunofluorescence microscopy. Immunoblotting. Immunoprecipitation. Immunohistochemistry on frozen paraformaldehyde-fixed sections: 1: 100. Immunohistochemistry on paraffin sections: 1: 100, 1h at RT, microwave treatmentrecommended. Immunohistochemistry on cytological material.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Liquid
注意事項 Avoid repeated freezing and thawing.
儲存條件 4 °C/-20 °C
儲存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

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