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更新時間:2017-12-28 14:18:48瀏覽次數(shù):441次
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抗原
Defensin, beta 1 (DEFB1)
別名 GAL3, CBD1, DEFB1, AW260221, BD-1, BD1, DEFB-1, DEFB101, HBD1, PBD-1, EBD, RHBD-1, DEFB1-like
適用
其他選擇 Human
Human(83),Murine (23),Rat ((Rattus)) (18),Pig (Porcine) (5),Wild boar (Sus scrofa) (5)
宿主
其他選擇 Rabbit
Rabbit(105),Mouse (7),Goat (2)
克隆類型
多克隆
標(biāo)記
其他選擇 Biotin
Biotin(16),FITC (7),HRP (5),Alexa Fluor 350 (2),Alexa Fluor 488 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),APC (1),Alkaline Phosphatase (AP) (1),PE (1)
應(yīng)用范圍
其他選擇 Enzyme Immunoassay (EIA), Western Blotting (WB)
Western Blotting (WB)(83),ELISA (68),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22),Immunohistochemistry (IHC) (21),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Immunocytochemistry (ICC) (13),Enzyme Immunoassay (EIA) (10),Flow Cytometry (FACS) (8),Immunohistochemistry (Frozen Sections) (IHC (fro)) (7),Immunofluorescence (IF) (6),Radioimmunoassay (RIA) (2),Blocking Antibody (Inhibition) (1),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1)
規(guī)格 50 μg
發(fā)貨至 中國 (更改)
發(fā)貨時間 29至35個工作日
DEFB1抗體
產(chǎn)品細(xì)節(jié)
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產(chǎn)品細(xì)節(jié)
顯示 隱藏
免疫原 E. Coli derived recombinant Human beta Defensin-1
特異性 This antibody detects Human BD-1. Other species not tested.
純化方法 Affinity Chromatography
目標(biāo)詳細(xì)情況
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目標(biāo)詳細(xì)情況
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別名 Defensin beta 1
背景 Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. The mature form of Beta defensin 1 is 36 amino acids.Synonyms: BD-1, BD1, Beta-defensin 1, DEFB1, DEFB101, HBD1, hBD-1
基因ID 1672
NCBI Accession NP_005209
UniProt P60022
研究領(lǐng)域 Immunology
使用細(xì)節(jié)
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使用細(xì)節(jié)
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應(yīng)用備注 ELISA: Direct: To detect Human BD-1 (using 100 μL/well antibody solution) a concentration of0.25-1.0?μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4?ng/well of recombinant Human BD-1. Sandwich: To detect Human BD-1 (using 100 μL/well antibody solution) a concentration of0.25-1.0?μg/mL of this antibody is required. In conjunction with Polyclonal Anti-Human BD-1(AP01151PU) as a capture antibody, it allows the detection of at least 0.2-0.4?ng/well ofrecombinant Human BD-1. Western blot: To detect Human BD-1 this antibody can be used at a concentration of 0.1-0.2?μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant Human BD-1 is 1.5-3.0?ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only
貯存及處理
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貯存及處理
顯示 隱藏
溶解方式 Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1?% BSA to a concentration of 0.1-1.0?mg/mL.
緩沖液 PBS,?pH 7.2 without preservatives.
儲存液 Without preservative
注意事項(xiàng) Avoid repeated freezing and thawing.
儲存條件 4 °C/-20 °C
儲存方法 Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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