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HIF1A抗體

抗原
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)
別名 hif1a, AA959795, HIF1alpha, MOP1, bHLHe78, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, PASD8, HIF1-alpha, hif-1a
抗原表位
其他選擇 AA 519-547, Center
AA 329-530(41),AA 432-528 (40),C-Term (24),AA 530-826 (11),AA 530-825 (9),AA 575-826 (8),Internal Region (8),AA 400-550 (7),AA 735-762 (7),N-Term (7),AA 350-400 (6),AA 400-450 (6),AA 550-600 (6),AA 700-750 (5),AA 1-30 (2),AA 215-336 (2),AA 45-180 (2),AA 588-836 (2),AA 641-785 (2),AA 717-826 (2),AA 728-757 (2),Middle Region (2),AA 1-31 (1),AA 1-735 (1),AA 218-506 (1),AA 220-349 (1),AA 238-389 (1),AA 328-377 (1),AA 35-155 (1),AA 519-547 (1),AA 520-550 (1),AA 585-824 (1),AA 635-660 (1),AA 641-810 (1),AA 657-826 (1),AA 775-790 (1),AA 776-826 (1),AA 809-826 (1),AA 817-821 (1),Lys377 (1),OH-Pro564 (1),hydroxyPro564 (1)
適用
其他選擇 Human, Murine
Human(265),Murine (111),Rat ((Rattus)) (76),Bovine (54),Pig (Porcine) (36),Monkey (31),Chicken (21),Dog (Canine) (16),Sheep (Ovine) (16),Ferret (15),Rabbit (10),Xenopus laevis (3),Hamster (2),Non-Human Primate (1),Primate (1),Protozoa (1),Wild boar (Sus scrofa) (1)
宿主
其他選擇 Rabbit
Rabbit(191),Mouse (128),Goat (2)
克隆類型 (克隆位點)
多克隆 (RB14548)
標(biāo)記
其他選擇 非結(jié)合性
Biotin(27),HRP (15),DyLight 488 (12),DyLight 550 (12),DyLight 650 (12),FITC (6),APC (3),Alkaline Phosphatase (AP) (3),PE (3),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Atto 390 (1),Atto 488 (1),Atto 565 (1),Atto 594 (1),Atto 633 (1),Atto 655 (1),Atto 680 (1),Atto 700 (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),HiLyte Fluor 488 (1),Magnetic Particles (1),PE-Atto 594 (1),PerCP (1),Sepharose (1),Streptavidin (1)
應(yīng)用范圍
其他選擇 Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
Western Blotting (WB)(239),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (101),ELISA (86),Immunohistochemistry (IHC) (83),Immunofluorescence (IF) (80),Immunocytochemistry (ICC) (63),Immunohistochemistry (Frozen Sections) (IHC (fro)) (46),Immunoprecipitation (IP) (40),Chromatin Immunoprecipitation (ChIP) (13),Flow Cytometry (FACS) (11),Gel Shift (GS) (10),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (8),Enzyme Immunoassay (EIA) (7),Electrophoretic Mobility-Shift Assay (EMSA) (4),Proximity Ligation Assay (PLA) (3),Immunoassay (IA) (1),Immunoelectron Microscopy (IEM) (1)
[Pubmed] 1 reference available
規(guī)格 400 μL
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HIF1A抗體

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產(chǎn)品細節(jié)

顯示 隱藏
免疫原 This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 AA from the Central region of human HIF1Alpha.
克隆位點 RB14548
亞型 Ig
特異性 This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic between amino acids 520-550 selected from the Central region of human HIF1Alpha.
預(yù)測反應(yīng) Cow (Bovine),Rat (Rattus)
純化方法 This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

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別名 HIF1Alpha
背景 Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.
Synonyms: Class E basic helix-loop-helix protein 78, bHLHe78, PAS domain-containing protein 8, Basic-helix-loop-helix-PAS protein MOP1, Member of PAS protein 1, ARNT-interacting protein, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha,PASD8, MOP1, BHLHE78, HIF1A
分子量 92670 DA
基因ID 3091
UniProt Q16665
研究領(lǐng)域 Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

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應(yīng)用備注 WB = 1:1000, IHC = 1:50-100, IF = 1:10-50, FACS = 1:10-50
限制 For Research Use only

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貯存及處理

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狀態(tài) Liquid
濃度 2 mg/mL
緩沖液 PBS with 0.09 % (W/V) sodium azide
儲存液 Sodium azide
注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
儲存條件 4 °C/-20 °C
儲存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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