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PLAA 抗體

抗原
Phospholipase A2-Activating Protein (PLAA)
別名 cb623, zgc:56568, zgc:66193, wu:fc45d08, wu:fi33e06, PLAA, doa1, pla2p, plap, DOA1, PLA2P, PLAP, 2410007N06, AI536418, AU018445, AW208417, D4Ertd618e, Ufd3, Plap
適用
其他選擇 Human
Human(48),Murine (3),Rat ((Rattus)) (3)
宿主
其他選擇 Rabbit
Rabbit(45),Mouse (3)
克隆類型
多克隆
標記
其他選擇 非結合性
FITC(4),Biotin (3),APC (2),Alkaline Phosphatase (AP) (2),HRP (2),PE (2)
應用范圍
其他選擇 Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB)(27),ELISA (25),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21),Immunohistochemistry (IHC) (18),Immunohistochemistry (Frozen Sections) (IHC (fro)) (12),Immunocytochemistry (ICC) (10),Immunofluorescence (IF) (4),Immunoprecipitation (IP) (2)
規(guī)格 0.5 mL
發(fā)貨至 中國 (更改)
發(fā)貨時間 43至57個工作日

PLAA 抗體

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

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免疫原 Recombinant protein encoding human placental alkaline phosphatase.
亞型 IgG
產(chǎn)品特性 Cellular Localization: Cytoplasm, Membrane
純化方法 Immunogen Affinity Purified

目標詳細情況

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別名 PLAP
背景 This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is expressed in testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
分子量 60-70 kDa
基因ID 250

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應用備注 IHC Dilution: 1:40
說明

Positive Control:
IHC (p): Placenta, seminoma

實驗流程 IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:40. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at RT.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
限制 For Research Use only

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狀態(tài) Liquid
緩沖液 immunogen affinity purified rabbit polyclonal antibody in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
儲存液 Sodium azide
注意事項 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事項 Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
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