Cadherin 12 抗體 返回列表頁(yè)

Cadherin 12 抗體

抗原
Cadherin 12
別名 Cdhb, RGD1566350, CDHB
抗原表位
其他選擇 AA 1-605
C-Term(10),N-Term (9),AA 1-605 (7),AA 69-98 (6),AA 695-724 (6),AA 688-716 (2),Internal Region (2),AA 1-794 (1),AA 372-470 (1),AA 62-90 (1),Middle Region (1)
適用
其他選擇 Human
Human(65),Murine (15),Rat ((Rattus)) (15),Chicken (1),Dog (Canine) (1)
宿主
其他選擇 Mouse
Rabbit(57),Mouse (8)
克隆類(lèi)型 (克隆位點(diǎn))
單克隆 (1)
標(biāo)記
其他選擇 非結(jié)合性
FITC(9),Biotin (7),HRP (7),PE (4),APC (2),Alkaline Phosphatase (AP) (2),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1)
zui低檢測(cè)濃度 0.078 ng/well
應(yīng)用范圍
其他選擇 ELISA
Western Blotting (WB)(49),ELISA (36),Immunohistochemistry (IHC) (33),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8),Flow Cytometry (FACS) (4),Enzyme Immunoassay (EIA) (2),Immunoassay (IA) (1),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1)
[Pubmed] 1 reference available
規(guī)格 50 μg
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Cadherin 12 抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
克隆位點(diǎn) 1
亞型 IgG1
特異性 Human Cadherin-12 / CDH12
交叉反應(yīng) （詳細(xì)） No cross-reactivity in ELISA with Human Cadherin-4 Human Cadherin-6 Human Cadherin-8 Human E-Cadherin Human cell lysate (293 cell line)
產(chǎn)品特性 This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cadherin-12 / CDH12 (rh Cadherin-12 / CDH12, NP_001783.2, Met 1-Ala 605). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
過(guò)濾 0.2 μm filtered

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 CDH12
背景 Classic Cadherins represent a family of calcium-dependent homophilic cell-cell adhesion molecules. They confer strong adhesiveness to animal cells when they are anchored to the actin cytoskeleton via their cytoplasmic binding partners, catenins. The cadherin/catenin adhesion system plays key roles in the morphogenesis and function of the vertebrate and invertebrate nervous systems. Furthermore, this system is involved in synaptic plasticity. Recent studies on the role of individual cadherin subtypes at synapses indicate that individual cadherin subtypes play their own unique role to regulate synaptic activities. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-12 also known as CDH12, is a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherin-12 appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.
NCBI Accession NP_001783.2

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA ELISA: 0.5-1 μg/mL This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CDH12. The detection limit for Human CDH12 is approximay 0.078 ng/well.
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲(chǔ)存液 Without preservative
注意事項(xiàng) Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲(chǔ)存條件 4 °C/-20 °C/-80 °C
儲(chǔ)存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
有效期 12 months

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