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HGF抗體

抗原
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF)
別名 HGF, C230052L06Rik, HGF/SF, NK1, NK2, SF/HGF, HPTA, DFNB39, F-TCF, HGFB, SF, AI838057, HGFR, Par4, c-Met
抗原表位
其他選擇 AA 1-728
AA 1-728(14),N-Term (13),C-Term (12),AA 521-554 (6),AA 529-559 (6),AA 495-728 (4),AA 32-728 (2),Middle Region (2),AA 1-342 (1),AA 28-494 (1),AA 306-471 (1),AA 32-291 (1),AA 482-710 (1),AA 619-728 (1),Internal Region (1)
適用
其他選擇 Human
Human(123),Murine (29),Rat ((Rattus)) (29),Dog (Canine) (17),Bovine (14),Guinea Pig (12),Pig (Porcine) (12),Fall Armyworm (Spodoptera frugiperda) (2),Rabbit (2),Chicken (1)
宿主
其他選擇 Rabbit
Rabbit(90),Mouse (34),Goat (6),Sheep (3)
克隆類型
多克隆
標(biāo)記
其他選擇 非結(jié)合性
Biotin(10),FITC (6),HRP (3),APC (2),Alkaline Phosphatase (AP) (2),PE (2),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),Magnetic Particles (1),Sepharose (1)
zui低檢測濃度 ≤ 0.039 ng/well
應(yīng)用范圍
其他選擇 ELISA
Western Blotting (WB)(97),ELISA (52),Immunohistochemistry (IHC) (33),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26),Immunocytochemistry (ICC) (13),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (10),Immunohistochemistry (Frozen Sections) (IHC (fro)) (9),Neutralization (Neut) (7),Enzyme Immunoassay (EIA) (5),Immunoprecipitation (IP) (5),Flow Cytometry (FACS) (4),Immunofluorescence (IF) (3),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (3),Immunoassay (IA) (1),Immunodiffusion (ID) (1),Immunoelectrophoresis (IEP) (1),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1),Proximity Ligation Assay (PLA) (1)
[Pubmed] 1 reference available
規(guī)格 100 μg
發(fā)貨至 中國 (更改)

HGF抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

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亞型 IgG
特異性 Human HGF
產(chǎn)品特性 Produced in rabbits immunized with purified, recombinant Human HGF (rh HGF, NP_000592.3, Met 1-Ser 728). Total IgG was purified by Protein A affinity chromatography.
純化方法 Affinity Purified
過濾 0.2 μm filtered

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 HGF
背景 Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
NCBI Accession NP_000592.3
研究領(lǐng)域 Cancer, Angiogenesis, Hormones, Growth Factors

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 ELISA ELISA: 0.5-1.0 μg/mL This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human HGF / Hepatocyte Growth Factor. The detection limit for Human HGF / Hepatocyte Growth Factor is approximay ≤ 0.039 ng/well.
限制 For Research Use only

貯存及處理

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貯存及處理

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緩沖液 PBS with 5% trehalose
儲存液 Without preservative
注意事項 Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
儲存條件 4 °C/-20 °C/-80 °C
儲存方法 This antibody can be stored at 2 °C-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C.
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