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IVL抗體

抗原
Involucrin (IVL)
別名 IVL, 1110019C06Rik
抗原表位
其他選擇 AA 420-578
AA 420-578(11),C-Term (4),N-Term (3),AA 301-460 (1),AA 421-568 (1),AA 461-551 (1),AA 50-142 (1),AA 536-585 (1),AA 6-156 (1),Pro568 (1)
適用
其他選擇 Human
Human(82),Dog (Canine) (13),Pig (Porcine) (12),Rat ((Rattus)) (10),Monkey (6),Gorilla (3),Owl (3),Murine (2),Bovine (1)
宿主
其他選擇 Rabbit
Rabbit(48),Mouse (41)
克隆類型
多克隆
標記
其他選擇 非結(jié)合性
Biotin(9),Alexa Fluor 350 (1),Alexa Fluor 488 (1),Alexa Fluor 555 (1),Alexa Fluor 647 (1),Cy3 (1),Cy5 (1),Cy5.5 (1),Cy7 (1),FITC (1),HRP (1),His tag (1),Magnetic Particles (1),Sepharose (1)
應用范圍
其他選擇 Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB)(61),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (54),ELISA (33),Immunoprecipitation (IP) (26),Immunofluorescence (IF) (21),Immunohistochemistry (IHC) (19),Immunohistochemistry (Frozen Sections) (IHC (fro)) (18),Immunocytochemistry (ICC) (15),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (9),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (7),Enzyme Immunoassay (EIA) (1),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1)
規(guī)格 0.1 mL
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發(fā)貨時間 29至35個工作日

IVL抗體

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

顯示 隱藏
免疫原 Recombinant protein encoding aa 420-578 of Human Involucrin .Remarks:
特異性 This antibody detects Involucrin (aa 420-578). Cellular localisation: Cytoplasmic.
純化方法 Affinity Chromatography

目標詳細情況

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目標詳細情況

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別名 Involucrin / IVL
背景 Involucrin is a soluble cytoplasmic protein precursor of the epidermal cornfied envelope that becomes cross-linked by transglutaminase during envelope assembly. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea which lacks a distinct cornfied layer. The protein is a useful marker of terminal differentiation. It is tightly linked to the onset of differentiation and first expressed in the immediate suprabasal layers of the epidermis.
分子量 120 kDa
基因ID 3713, 9606
UniProt P07476

使用細節(jié)

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使用細節(jié)

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應用備注 Western blot: 1/50 for 1 hour at RT. Immunohistochemistryon on Paraffin-Embedded Tissues: 1/100 for 10?min at RT. staining offormalin-fixed tissues requires boiling tissue sections in 10?mM citrate buffer,?pH 6.0 for 10?min followed by cooling at RT for 20?min. Incubation Time: 10?minutes at RT. Slides must be washed in between steps. Rinse slides with PBS/0.05?% Tween. Positive control: Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 For Research Use only

貯存及處理

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貯存及處理

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狀態(tài) Liquid
緩沖液 10?mM PBS,?pH 7.4 containing 1?% BSA as stabilizer and 0.09?% Sodium Azide as preservative
儲存液 Sodium azide
注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
儲存條件 4 °C
儲存方法 Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

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