E2F1抗體 返回列表頁

E2F1抗體

抗原
E2F Transcription Factor 1 (E2F1)
別名
CG6376, DRTF1/E2F, DmE2F-1, DmelCG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f1, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)0 ... show more
CG6376, DRTF1/E2F, DmE2F-1, DmelCG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f1, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729, RBAP1, RBBP3, RBP3, mKIAA4009, xE2F, ATE2FB, E2F TRANSCRIPTION FACTOR-1 E2F1, E2F transcription factor 1, E2FB, E2FB TRANSCRIPTION FACTOR show less
抗原表位
其他選擇 AA 69-223
pSer337(30),pSer364 (24),pThr433 (23),pSer332 (17),Ser332 (15),Internal Region (11),acLys120 (7),acLys125 (7),AA 162-476 (6),AA 360-369 (6),AA 430-434 (6),N-Term (6),Ser337 (6),AA 244-264 (5),C-Term (5),AA 223-332 (4),Middle Region (4),AA 332-390 (3),His357 (3),pHis357 (3),AA 133-322 (2),AA 133-362 (2),AA 300-350 (2),AA 310-329 (2),AA 392-414 (2),AA 138-144 (1),AA 183-433 (1),AA 223-322 (1),AA 310-344 (1),AA 314-327 (1),AA 342-371 (1),AA 348-437 (1),AA 387-437 (1),AA 388-437 (1),AA 43-217 (1),AA 480-493 (1),AA 58-83 (1),AA 69-223 (1),Asp426 (1),Ser310 (1),Thr433 (1)
適用
其他選擇 Human, Murine
Human(274),Murine (95),Rat ((Rattus)) (58),Bovine (27),Dog (Canine) (27),Pig (Porcine) (25),Guinea Pig (12),Chimpanzee (2),Chicken (1),Fruit Fly (Drosophila melanogaster) (1),Monkey (1),Xenopus laevis (1)
宿主
其他選擇 Mouse
Rabbit(226),Mouse (48),Goat (3),Sheep (1)
克隆類型 (克隆位點(diǎn))
單克隆 (8G9)
標(biāo)記
其他選擇 非結(jié)合性
Biotin(15),FITC (12),HRP (11),Alexa Fluor 350 (6),Alexa Fluor 488 (6),Alexa Fluor 555 (6),Alexa Fluor 647 (6),Cy3 (6),Cy5 (6),Cy5.5 (6),Cy7 (6),APC (5),Alkaline Phosphatase (AP) (5),PE (5)
應(yīng)用范圍
其他選擇 ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
Western Blotting (WB)(196),ELISA (83),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (60),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (48),Immunoprecipitation (IP) (44),Immunohistochemistry (IHC) (41),Dot Blot (DB) (24),Immunofluorescence (IF) (24),Enzyme Immunoassay (EIA) (12),Immunocytochemistry (ICC) (8),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (7),Gel Shift (GS) (5),Flow Cytometry (FACS) (3),Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (2),Immunohistochemistry (Frozen Sections) (IHC (fro)) (2),Proximity Ligation Assay (PLA) (2)
[Pubmed] 3 references available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時(shí)間 24至29個(gè)工作日

E2F1抗體

產(chǎn)品細(xì)節(jié)

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產(chǎn)品細(xì)節(jié)

顯示 隱藏
免疫原 Purified recombinant fragment of human E2F1 (AA 69-223) expressed in E. coli.
克隆位點(diǎn) 8G9
亞型 IgG1
純化方法 Purified

目標(biāo)詳細(xì)情況

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目標(biāo)詳細(xì)情況

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別名 E2F1
背景 Synonyms:
RBP3, E2F-1, RBAP1, RBBP3
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic AA, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
分子量 46.9 kDa
基因ID 1869
研究領(lǐng)域 Cancer, Transcription Factors

使用細(xì)節(jié)

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使用細(xì)節(jié)

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應(yīng)用備注 Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
限制 For Research Use only

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貯存及處理

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狀態(tài) Liquid
緩沖液 PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
儲(chǔ)存液 Sodium azide
注意事項(xiàng) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
儲(chǔ)存條件 4 °C/-20 °C
儲(chǔ)存方法 Store at 4 °C or at -20 °C for long term.

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