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AFP抗體 

抗原
alpha-Fetoprotein (AFP)
別名 FETA, HPAFP
適用
其他選擇 Human, Murine
Human(569),Murine (40),Rat ((Rattus)) (36),Dog (Canine) (10),Pig (Porcine) (9),Bovine (3),Wild boar (Sus scrofa) (3),Mammalian (2),Monkey (1),Sheep (Ovine) (1)
宿主
其他選擇 Mouse
Mouse(347),Rabbit (219),Goat (34),Chicken (6),Sheep (5),Duck (2)
克隆類型 (克隆位點)
單克隆 (C2)
標記
其他選擇 非結合性
Biotin(22),HRP (19),FITC (15),Alexa Fluor 350 (5),Alexa Fluor 488 (5),Alexa Fluor 555 (5),Alexa Fluor 647 (5),Cy3 (5),Cy5 (5),Cy5.5 (5),Cy7 (5),PE (3),APC (2),Alkaline Phosphatase (AP) (2),Magnetic Particles (1),Sepharose (1)
應用范圍
其他選擇 Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB)(285),ELISA (267),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (132),Immunohistochemistry (IHC) (82),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (50),Immunohistochemistry (Frozen Sections) (IHC (fro)) (49),Flow Cytometry (FACS) (47),Immunofluorescence (IF) (43),Immunoprecipitation (IP) (36),Enzyme Immunoassay (EIA) (25),Immunocytochemistry (ICC) (25),Radioimmunoassay (RIA) (24),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (19),Lateral Flow (LF) (8),ELISA (Capture) (7),Protein Assay (PrA) (7),Immunoassay (IA) (6),Immunodiffusion (ID) (6),Immunoelectrophoresis (IEP) (6),ELISA (Detection) (5),Immunohistochemistry (Acetone-fixed) (IHC (af)) (5),Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (5),Detection (D) (3),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2),Labeling (Lbl) (2),Dot Blot (DB) (1),Gel Diffusion (GD) (1),Neutralization (Neut) (1),Radial Immunodiffusion (RID) (1)
[Pubmed] 1 reference available
規(guī)格 0.1 mg
發(fā)貨至 中國 (更改)
發(fā)貨時間 29至35個工作日

AFP抗體 

產(chǎn)品細節(jié)

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產(chǎn)品細節(jié)

顯示 隱藏
免疫原 A BALB/c mouse has been immunized with alpha fetoprotein isolated from serum of hepatoma patients. Splenocytes were fused with mouse myeloma cell line P3-X63-Ag8.653.
克隆位點 C2
亞型 IgG1
特異性 C2 is characterized in the ISOBM TD-2 workshop and assigned by K. Nustad to group E of a cluster of 6 major epitopes of Human Alpha Fetoprotein. C2 specifically reacts with Human and Mouse AFP, but does not react with Rat, Dog or calf Alpha Fetoprotein as demonstrated by ELISA.
交叉反應 （詳細） Species reactivity (tested):Human, Mouse.
純化方法 Purified

 目標詳細情況

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目標詳細情況

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背景 Human alpha fetoprotein is an oncofetal protein of 70 kD. Its gene is cloned and sequenced and its structure identified. AFP is expressed in fetal liver and is normally absent in healthy adult tissues. It is expressed in all yolk sac tumors, in some germ cell tumors and in hepatocellular carcinomas. Alpha Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. alpha Fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha Fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha Fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha Fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha Fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP
基因ID 174
NCBI Accession NP_001125
UniProt P02771
研究領域 Serum/Plasma Proteins

 使用細節(jié)

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使用細節(jié)

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應用備注 Optimal working dilution should be determined by the investigator.
限制 For Research Use only

貯存及處理

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貯存及處理

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濃度 0,1 mg/mL
緩沖液 PBS, 0.05?% Sodium Azide
儲存液 Sodium azide
注意事項 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
儲存條件 4 °C

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