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磷酸化沉默調(diào)節(jié)蛋白1抗體

英文名稱(chēng) phospho-SIRT1 (Thr530)
中文名稱(chēng) 磷酸化沉默調(diào)節(jié)蛋白1抗體
別    名 75SirT1; hSIR2; hSIRT1; HST2, S. cerevisiae, homolog of; NAD dependent deacetylase sirtuin 1; NAD dependent protein deacetylase sirtuin 1; OTTHUMP00000198111; OTTHUMP00000198112; Regulatory protein SIR2 homolog 1; SIR1_HUMAN; SIR2 like 1; SIR2 like protein 1; SIRT1 (phospho T530); p-SIRT1 (phospho T530); SIR2, S.cerevisiae, homolog-like 1; SIR2-like protein 1; SIR2ALPHA; SIR2L1; Sirt1; SirtT1 75 kDa fragment; Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); Sirtuin 1; Sirtuin type 1.
   
說(shuō) 明 書(shū) 0.1ml  
產(chǎn)品類(lèi)型 磷酸化抗體 
研究領(lǐng)域 細(xì)胞生物  細(xì)胞凋亡  細(xì)胞類(lèi)型標(biāo)志物  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human, 
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 81kDa
細(xì)胞定位 細(xì)胞漿 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human SIRT1 around the phosphorylation site of Thr530
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產(chǎn)品介紹 background:
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Subcellular Location:
Cytoplasm. Mitochondrion and Nucleus > PML body. Cytoplasm. Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm.

Tissue Specificity:
Widely expressed.

Post-translational modifications:
Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53.
Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 activates deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity). Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545.
Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1).
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.xy-2272R    phospho-ATM(Ser1981)磷酸化毛細(xì)血管擴(kuò)張性共濟(jì)失調(diào)癥突變蛋白抗體
xy-3037R    phospho-ATP citrate lyase (Ser455)磷酸化三磷酸腺檸檬酸裂解酶抗體
xy-3030R    phospho-ATR (Ser428)磷酸化ATR抗體
xy-7960R    AMN1細(xì)胞核重定位及紡錘體檢查點(diǎn)蛋白AMN1抗體
xy-7957R    ALG11天門(mén)冬酰胺連接糖基化11抗體
xy-3777R    ASF1A細(xì)胞衰老相關(guān)蛋白ASF1A抗體
xy-8457R    AGPHD1氨基糖苷類(lèi)磷酸結(jié)構(gòu)域蛋白抗體
xy-4119R    ASGPR1唾液酸糖蛋白受體1抗體
xy-9100R    ARSF芳香基硫酸酯酶F抗體
xy-6980R    AKAP5A激酶錨定蛋白5抗體
xy-4807R    ATXN3L小腦脊髓共濟(jì)失調(diào)蛋白3抗體
xy-9297R    ASTE1ASTE1蛋白抗體
xy-5212R    phospho-APG4B(Ser309)磷酸化自噬相關(guān)蛋白4B抗體
xy-9790R    AFAP微絲相關(guān)蛋白1抗體
xy-4235R    ADORA1腺苷A1A受體抗體
xy-4231R    ARSB芳基硫酸酯酶B抗體
xy-4234R    ABATγ氨基丁酸轉(zhuǎn)氨酶抗體
xy-6513R    A2BP1共濟(jì)失調(diào)蛋白2結(jié)合蛋白1抗體
xy-4236R    ADAM17腫瘤壞死因子α轉(zhuǎn)換酶
xy-4238R    ARNT2 芳香烴受體核轉(zhuǎn)錄蛋白2抗體
xy-6510R    ABH8AlkB同源蛋白8抗體
xy-6506R    ABI3BPABI基因家族成員3結(jié)合蛋白抗體
xy-4271R    ARHGAP20Rho GTP酶激活蛋白20抗體
xy-4653R    ASB10含錨蛋白重復(fù)序列-細(xì)胞因子信號(hào)抑制物盒蛋白家族10抗體
xy-5709R    ALDH1L1乙醛脫氫酶1蛋白家族L1抗體
xy-5717R    Axin 2軸抑制蛋白2抗體
xy-7027R    ANGPTL6血管生成素相關(guān)蛋白6抗體
xy-4687R    AMHMuellerian繆勒管激素抑制因子抗體
xy-7035R    EphA8酪氨酸蛋白激酶受體A8抗體
xy-11383R    Acetoacetyl-CoA synthetase脂肪酰輔酶A家族成員1抗體
xy-11446R    ARNTL2芳香烴受體核轉(zhuǎn)錄蛋白樣2抗體
xy-11448R    phospho-AANAT (Thr29)磷酸化芳香胺N-乙?；D(zhuǎn)移酶抗體
xy-11449R    ABLIM1肌動(dòng)蛋白結(jié)合蛋白1抗體
xy-11450R    AMIGO2粘附分子IgG樣結(jié)構(gòu)域蛋白2抗體
xy-11451R    Advillin肌動(dòng)蛋白結(jié)合蛋白DOC6抗體