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乳腺癌NY BR 85抗原抗體

英文名稱 Shugoshin
中文名稱 乳腺癌NY BR 85抗原抗體
別    名 hSgo 1; hSgo1; NY BR 85; Serologically defined breast cancer antigen NY BR 85; Serologically defined breast cancer antigen NY-BR-85; SGO 1; SGO; SGO L1; SGO1; SGOL 1; SGOL1; SGOL1_HUMAN; Shugoshin 1AB protein; Shugoshin 1CD protein; Shugoshin 1EFprotein; Shugoshin 1GH protein; Shugoshin 1KL protein; Shugoshin like 1 (S. pombe); Shugoshin like 1; Shugoshin-like 1.
   
說 明 書 0.1ml  0.2ml  
研究領(lǐng)域 腫瘤  細(xì)胞生物  細(xì)胞分化  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Rat, Pig, Horse, 
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 64kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human Shugoshin
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產(chǎn)品介紹 background:
Sgo1 is a 561 amino acid nuclear and cytoplasmic protein that is widely expressed with highest expression in testis. Sgo1 localizes to the inner centromere throughout prophase until metaphase. Sgo1 is suggested to prevent premature dissociation of the cohesin complex from centromeres after prophase by impeding phosphorylation of the SA-2 subunit of the cohesin complex at the centromere. This ensures cohesin persistence at the centromere until cohesin cleavage is achieved by Separase at the anaphase stage of mitosis. Sgo1 is essential for proper chromosome segregation and for proper attachment of spindle microtubule to the kinetochore. Sgo1 may also play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating Plk kinetochore affinity. Sgo1 exists as seven alternatively isoforms one of which (isoform 3) does not does not localize to kinetochores during any stages of the cell cycle

Function:
Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.