TFF2抗原；三葉因子2(TFF2)重組蛋白多種屬 返回列表頁(yè)

Recombinant Trefoil Factor 2 (TFF2) 

三葉因子2(TFF2)重組蛋白

[ PROPERTIES ]

Residues:Ser27~His128,with N-terminal His-Tag.

Host: E.coli

Subcellular Location: Secreted

Purity: > 95%

Endotoxin Level:<1.0EU per 1μg(determined by the LAL method).

Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point:5.9

Predicted Molecular Mass: 13.0kDa

Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducingconditions.

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note:The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.

MGHHHHHHSGSEF-SPCQ CSRLSPHNRT NCGFPGITSD QCFDNGCCFD SSVTGVPWCFHPLPKQESDQ CVMEVSDRRN CGYPGISPEE CASRKCCFSN FIFEVPWCFF PKSVEDCH

[  IDENTIFICATION  ]

特別提示:本公司的所有產(chǎn)品僅可用于科研實(shí)驗(yàn),嚴(yán)禁用于臨床醫(yī)療及其他非科研用途!以實(shí)際收貨產(chǎn)品說(shuō)明書為準(zhǔn),網(wǎng)站說(shuō)明書僅供參考！