目錄:愛(ài)必信（上海）生物科技有限公司>>細(xì)胞生物學(xué)>>重組蛋白>> abs04184β-NGF （Ser122-Ala241, E. coli）（10ug）
| 參考價(jià) | ￥ 400 |
| 訂貨量 | ≥1 |
| ￥400 |
| ≥1 |
更新時(shí)間：2024-07-08 09:27:43瀏覽次數(shù)：688評(píng)價(jià)
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| 供貨周期 | 一周 | 規(guī)格 | 10ug |
|---|---|---|---|
| 貨號(hào) | abs04184 |
| 概述 | |
|---|---|
| 描述 | Recombinant Human beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Ser122-Ala241 is expressed. |
| 別名 | Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB |
| 形態(tài) | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.0. |
| 來(lái)源 | E.coli |
| 氨基酸序列 | SSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVD SGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVRRA |
| 內(nèi)毒素水平 | Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Accession # | P01138 |
| 性能 | |
|---|---|
| 背景 | Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α, β, and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity. |
| 溶解方法 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 保存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 純度 | Greater than 95% as determined by reducing SDS-PAGE. |
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