目錄:愛必信（上海）生物科技有限公司>>細(xì)胞生物學(xué)>>重組蛋白>> abs04177IGF-I/IGF1（10ug）
| 參考價(jià) | ￥ 200 |
| 訂貨量 | ≥1 |
| ￥200 |
| ≥1 |
更新時(shí)間：2024-07-08 10:13:42瀏覽次數(shù)：1032評(píng)價(jià)
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| 供貨周期 | 一周 | 規(guī)格 | 10ug |
|---|---|---|---|
| 貨號(hào) | abs04177 |
| 概述 | |
|---|---|
| 描述 | Recombinant Human Insulin-like Growth Factor I is produced by our E.coli expression system and the target gene encoding Gly49-Ala118 is expressed. |
| 別名 | Insulin-Like Growth Factor I, IGF-I, Mechano Growth Factor, MGF, Somatomedin-C, IGF1, IBP1 |
| 形態(tài) | Lyophilized from a 0.2 μm filtered solution of 300mM NaAc, pH 6.5. |
| 來源 | E.coli |
| 氨基酸序列 | GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLK PAKSA |
| 內(nèi)毒素水平 | Less than 0.05 ng/µg (0.5 IEU/µg) as determined by LAL test. |
| Accession # | P05019 |
| 性能 | |
|---|---|
| 背景 | Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contains the N- and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids has 94% identity with mouse IGF-I and exhibits cross-species activity. IGF-1 binds IGF-IR, IGF-IIR, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone. R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. R3 IGF-1 has been produced with the purpose of increasing biological activity. R3 IGF-1 is significantly more potent than human IGF-I in vitro. |
| 溶解方法 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 500mM Acetic Acid. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 保存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 純度 | Greater than 95% as determined by reducing SDS-PAGE. |
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